PMID: 9557757Apr 29, 1998Paper

Identification of hepatitis G virus particles in human serum by E2-specific monoclonal antibodies generated by DNA immunization

Journal of Virology
S SchmolkeB Ofenloch-Haehnle

Abstract

In order to elucidate the structure and morphology of hepatitis G virus (HGV), a recently isolated flavivirus, we generated a panel of eight monoclonal antibodies (MAbs) against the putative second envelope protein (E2) following DNA immunization. The MAbs were shown to be specific for four different epitopes on recombinant E2. MAb Mc6 was the only antibody able to detect the linear epitope LTGGFYEPL. In addition, Mc6 was able to immunoprecipitate viral particles in human blood samples as detected by reverse transcription-PCR amplification of HGV RNA. This precipitation could be competed by addition of saturating amounts of the linear peptide or abolished by addition of Nonidet P-40. We conclude that, albeit lacking the N-terminal sequence of a functional core protein, HGV builds classical viral particles displaying E2 envelope protein on their outer surfaces.

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Citations

Jul 28, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·José-Francisco GarcíaGiovanna Roncador
Sep 18, 2009·Journal of Viral Hepatitis·Emma L Mohr, Jack T Stapleton
Sep 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emma L MohrJack T Stapleton
Apr 7, 2016·Emerging Microbes & Infections·Shuying LiuShan Lu

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