Abstract
In order to elucidate the structure and morphology of hepatitis G virus (HGV), a recently isolated flavivirus, we generated a panel of eight monoclonal antibodies (MAbs) against the putative second envelope protein (E2) following DNA immunization. The MAbs were shown to be specific for four different epitopes on recombinant E2. MAb Mc6 was the only antibody able to detect the linear epitope LTGGFYEPL. In addition, Mc6 was able to immunoprecipitate viral particles in human blood samples as detected by reverse transcription-PCR amplification of HGV RNA. This precipitation could be competed by addition of saturating amounts of the linear peptide or abolished by addition of Nonidet P-40. We conclude that, albeit lacking the N-terminal sequence of a functional core protein, HGV builds classical viral particles displaying E2 envelope protein on their outer surfaces.
References
Jan 1, 1992·Medical Microbiology and Immunology·R ThomssenA Uy
Mar 23, 1990·Science·J A WolffP L Felgner
Jan 1, 1990·Annual Review of Microbiology·T J ChambersC M Rice
Jan 1, 1981·Methods in Enzymology·G Galfrè, C Milstein
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·M ShindoS M Feinstone
Nov 1, 1993·Human Molecular Genetics·H L DavisR G Whalen
Apr 1, 1993·Journal of Virology·M HijikataH Yoshikura
Jan 26, 1996·Science·J LinnenJ P Kim
Jun 6, 1996·The New England Journal of Medicine·K MasukoM Mayumi
May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·E RazD A Carson
Sep 1, 1996·Journal of Virology·T M PertmerJ R Haynes
Sep 1, 1996·Journal of Virology·J N SimonsI K Mushahwar
Jan 1, 1996·Journal of Medical Virology·T P LearyI K Mushahwar
Nov 1, 1996·Journal of Clinical Microbiology·V SchlueterA M Engel
Nov 15, 1996·Virology·T J Pilot-MatiasI K Mushahwar
Dec 1, 1996·The Journal of Infectious Diseases·K StarkV Schluter
Dec 24, 1996·Biochemical and Biophysical Research Communications·K SatoM Mayumi
Jan 1, 1997·Journal of Virology·V DeleersnyderJ Dubuisson
Feb 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·V TedeschiS M Feinstone
Feb 1, 1997·Lancet·M TackeA M Engel
Mar 13, 1997·The New England Journal of Medicine·H J AlterJ P Kim
Feb 1, 1997·The Journal of Infectious Diseases·B J DilleI K Mushahwar
Nov 21, 1997·The Journal of General Virology·M Y LimJ P Kim
Dec 16, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M TackeA M Engel
Citations
Jul 28, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·José-Francisco GarcíaGiovanna Roncador
Oct 13, 2006·Journal of Virology·James H McLindenJack T Stapleton
Sep 18, 2009·Journal of Viral Hepatitis·Emma L Mohr, Jack T Stapleton
Jan 16, 1999·Hybridoma·K E KilpatrickJ T Hutchins
Sep 10, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emma L MohrJack T Stapleton
Apr 7, 2016·Emerging Microbes & Infections·Shuying LiuShan Lu
Apr 10, 1999·Journal of Virology·M FogedaV Carreño