Identification of hepatocellular carcinoma-associated hub genes and pathways by integrated microarray analysis

Tumori
Fangfeng LiuJun Lu

Abstract

Hepatocellular carcinoma (HCC) is a dismal malignancy associated with multiple molecular changes. The purpose of this study was to identify the differentially expressed genes and analyze the biological processes related to HCC. Datasets of HCC were obtained from the NCBI Gene Expression Omnibus. Integrated analysis of differentially expressed genes was performed using the INMEX program. Then Gene Ontology enrichment analyses and pathway analysis were performed based on the Gene Ontology website and Kyoto Encyclopedia of Genes and Genomes. A protein-protein interaction network was constructed using the Cytoscape software; the netwerk served to find hub genes for HCC. Real-time RT-PCR was used to validate the microarray data for hub genes. We identified 273 genes that were differentially expressed in HCC. Gene Ontology enrichment analyses revealed response to cadmium ion, cellular response to cadmium ion, and cellular response to zinc ion for these genes. Pathway analysis showed that significant pathways included fatty acid metabolism, butanoate metabolism, and PPAR signaling pathway. The protein-protein interaction network indicated that CDH1, ECHS1, ACAA1, MT2A, and MYC were important genes which participated in many interactio...Continue Reading

References

Jan 1, 1992·The Prostate·D J CulkinR A Zitman
Feb 13, 1999·Nature Genetics·K J WuR Dalla-Favera
Jun 23, 1999·Oncogene·C E NesbitE V Prochownik
Jul 5, 2003·Genes & Development·Carla GrandoriRaymond J Monnat
Sep 25, 2004·Radiation Research·Lynda FletcherRuth J Muschel
Aug 9, 2005·Cancer Letters·Daniel G Hayward, Andrew M Fry
Sep 15, 2005·The Journal of Biological Chemistry·Wei ZhangRuth J Muschel
Nov 12, 2005·Molecular and Cellular Biochemistry·Min Ok Song, Jonathan H Freedman
Feb 4, 2006·Journal of Biomedical Science·Li MaXueliang Zhu
May 9, 2006·Acta Biochimica Et Biophysica Sinica·Faiz M M T MarikarZi-Chun Hua
Jan 6, 2007·International Journal of Cancer. Journal International Du Cancer·Sallyann L O'BrienWilliam M Gallagher
Feb 24, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hu-Liang JiaXin Wei Wang
Jun 16, 2007·Gastroenterology·Hashem B El-Serag, K Lenhard Rudolph
Jun 30, 2007·Current Biology : CB·Maïlys A S Vergnolle, Stephen S Taylor
Feb 18, 2009·The Journal of Clinical Investigation·Byoung Kwon YooDevanand Sarkar
Feb 20, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Sean F AltekruseMarsha E Reichman
Apr 9, 2009·Expert Review of Gastroenterology & Hepatology·Yujin HoshidaJosep M Llovet
May 1, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Brad Q StarleyStephen A Harrison
Mar 2, 2011·Lipids·Subhashis BanerjeeTodd D Porter
Apr 28, 2012·Gastroenterology·Hashem B El-Serag
Feb 19, 2013·FEBS Letters·Yan ChangWei-Na Zhang
Feb 22, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Yuji HodoShuichi Kaneko
Jun 15, 2013·Nucleic Acids Research·Jianguo XiaRobert E W Hancock
Jul 25, 2013·Critical Reviews in Eukaryotic Gene Expression·Xiao-san ZhuQiu-hui Tan
Feb 22, 2014·American Journal of Alzheimer's Disease and Other Dementias·Bingqian DingWeiya He
Feb 25, 2014·The Journal of Surgical Research·Curtis J WrayFilemon K Tan

❮ Previous
Next ❯

Citations

Feb 26, 2016·Metabolism: Clinical and Experimental·Heinz Zoller, Herbert Tilg
Mar 19, 2016·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Qinghua XuXiang Du
May 31, 2018·Cancer Biomarkers : Section a of Disease Markers·Liang ZhangYi Zhu
Sep 20, 2018·Canadian Journal of Gastroenterology & Hepatology·Ozlem KutluEbru Ozer
May 12, 2016·World Journal of Hepatology·Venerando RapisardaGaetano Bertino
Mar 16, 2017·Current Diabetes Reports·P WainwrightC D Byrne
Jul 9, 2021·Frontiers in Genetics·Yanli WangYang Cao

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE6222
GSE41804

Methods Mentioned

BETA
biopsy
PCR

Software Mentioned

INMEX
R
Cytoscape

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.