Identification of heterochronic mutants in Caenorhabditis elegans. Temporal misexpression of a collagen::green fluorescent protein fusion gene.

Genetics
J E AbrahanteA E Rougvie

Abstract

The heterochronic genes lin-4, lin-14, lin-28, and lin-29 specify the timing of lateral hypodermal seam cell terminal differentiation in Caenorhabditis elegans. We devised a screen to identify additional genes involved in this developmental timing mechanism based on identification of mutants that exhibit temporal misexpression from the col-19 promoter, a downstream target of the heterochronic gene pathway. We fused the col-19 promoter to the green fluorescent protein gene (gfp) and demonstrated that hypodermal expression of the fusion gene is adult-specific in wild-type animals and temporally regulated by the heterochronic gene pathway. We generated a transgenic strain in which the col-19::gfp fusion construct is not expressed because of mutation of lin-4, which prevents seam cell terminal differentiation. We have identified and characterized 26 mutations that restore col-19::gfp expression in the lin-4 mutant background. Most of the mutations also restore other aspects of the seam cell terminal differentiation program that are defective in lin-4 mutant animals. Twelve mutations are alleles of three previously identified genes known to be required for proper timing of hypodermal terminal differentiation. Among these are four ne...Continue Reading

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Citations

Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·G D Hayes, G Ruvkun
Sep 5, 2001·Nature Reviews. Genetics·A E Rougvie
Nov 23, 2005·Developmental Biology·Jason M TennessenAnn E Rougvie
May 4, 2002·Nature Reviews. Genetics·Erik M Jorgensen, Susan E Mango
Mar 6, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Melanie C TheinAntony P Page
Mar 17, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Tamar D ResnickAnn E Rougvie
Dec 6, 2011·Current Biology : CB·Gabriela C MonsalveAlison R Frand
Aug 12, 2010·PLoS Genetics·Katherine Olsson-Carter, Frank J Slack
Feb 10, 2007·Genes & Development·Stephen E Von StetinaDavid M Miller
Mar 11, 2011·Annals of the New York Academy of Sciences·Liesbet TemmermanLiliane Schoofs
May 26, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Rebecca J AndrowskiNathan E Schroeder
Aug 5, 2011·PLoS Genetics·Daniel HochbaumAlfred L Fisher
Oct 13, 2011·Developmental Biology·Sascha RusselGary Ruvkun

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