PMID: 9445045Jan 28, 1998Paper

Identification of highly attenuated mutants of simian immunodeficiency virus

Journal of Virology
R C DesrosiersR P Johnson

Abstract

Deletion mutants of the pathogenic clone of simian immunodeficiency virus isolate 239 (SIVmac239) were derived that are missing nef, vpr, and upstream sequences (US) in the U3 region of the LTR (SIVmac239 delta3), nef, vpx, and US (SIVmac239 delta3x), and nef, vpr, vpx, and US (SIVmac239 delta4). These multiply deleted derivatives replicated well in the continuously growing CEMx174 cell line and were infectious for rhesus monkeys. However, on the basis of virus load measurements, strength of antibody responses, and lack of disease progression, these mutants were highly attenuated. Measurements of cell-associated viral load agreed well with assays of plasma viral RNA load and with the strengths of the antibody responses; thus, these measurements likely reflected the extent of viral replication in vivo. A derivative of SIVmac239 lacking vif sequences (SIVmac239 delta vif) could be consistently grown only in a vif-complementing cell line. This delta vif virus appeared to be very weakly infectious for rhesus monkeys on the basis of sensitive antibody tests only. The weak antibody responses elicited by SIVmac239 delta vif were apparently in response to low levels of replicating virus since they were not elicited by heat-inactivated ...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Nov 1, 1990·AIDS Research and Human Retroviruses·D A Regier, R C Desrosiers
Dec 1, 1987·The Journal of General Virology·M D DanielR C Desrosiers
Jun 7, 1985·Science·M D DanielR C Desrosiers
Jan 26, 1995·The New England Journal of Medicine·F KirchhoffR C Desrosiers
Apr 1, 1995·Journal of Virology·J S GibbsR C Desrosiers
May 1, 1994·AIDS Research and Human Retroviruses·J S GibbsR C Desrosiers
Feb 1, 1994·AIDS Research and Human Retroviruses·M G LewisG A Eddy
Jun 1, 1996·Journal of Virology·M S WyandR C Desrosiers
Oct 6, 1997·Journal of Virology·R E MeansR C Desrosiers

❮ Previous
Next ❯

Citations

Jun 12, 1998·Nature Medicine·J N ReitterR C Desrosiers
Sep 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M RosenzweigA A Lackner
Feb 13, 2002·AIDS Research and Human Retroviruses·Stephen J KentDamian F J Purcell
Jan 31, 2002·Annual Review of Medicine·Welkin E Johnson, Ronald C Desrosiers
Mar 23, 2005·The Journal of Biological Chemistry·Masayuki KobayashiTakashi Uchiyama
Mar 25, 2006·Uirusu·Akifumi Takaori
Nov 23, 2006·The Journal of Experimental Medicine·Marie-Claire GauduinR Paul Johnson
Jun 25, 2008·International Reviews of Immunology·Takeo Ohsugi, Atsushi Koito
Dec 30, 2009·The Journal of Experimental Medicine·Nicoletta CasartelliArnaud Moris
Dec 2, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nadine C SalischR Paul Johnson
Aug 2, 2013·Viruses·Stefán R Jónsson, Valgerdur Andrésdóttir
Jan 28, 2014·PloS One·Susan V WestmorelandRonald C Desrosiers
Mar 30, 2001·The Journal of Biological Chemistry·G HassaïneE Decroly
Jun 2, 2004·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·Jane B GrecoR Gilberto González
Jul 16, 2004·Journal of Virology·Masayuki KobayashiTakashi Uchiyama
Jan 31, 2002·AIDS·P J Bock, D M Markovitz
Dec 12, 2001·Journal of Virology·Agneta S von GegerfeltBarbara K Felber
Jul 10, 2009·Journal of Virology·Fransje A KoningMichael H Malim

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.