Identification of host proteins required for HIV infection through a functional genomic screen

Science
Abraham L BrassStephen J Elledge

Abstract

HIV-1 exploits multiple host proteins during infection. We performed a large-scale small interfering RNA screen to identify host factors required by HIV-1 and identified more than 250 HIV-dependency factors (HDFs). These proteins participate in a broad array of cellular functions and implicate new pathways in the viral life cycle. Further analysis revealed previously unknown roles for retrograde Golgi transport proteins (Rab6 and Vps53) in viral entry, a karyopherin (TNPO3) in viral integration, and the Mediator complex (Med28) in viral transcription. Transcriptional analysis revealed that HDF genes were enriched for high expression in immune cells, suggesting that viruses evolve in host cells that optimally perform the functions required for their life cycle. This effort illustrates the power with which RNA interference and forward genetics can be used to expose the dependencies of human pathogens such as HIV, and in so doing identify potential targets for therapy.

References

Nov 26, 1986·Biochemical and Biophysical Research Communications·H A BloughL Thiry
Oct 6, 1998·Annual Review of Biochemistry·A D Frankel, J A Young
Sep 16, 2000·The EMBO Journal·S SiniossoglouH R Pelham
Oct 16, 2002·The Journal of Biological Chemistry·Symeon Siniossoglou, Hugh R B Pelham
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Eiji Morita, Wesley I Sundquist
Apr 15, 2005·Molecular Biology of the Cell·Judith A ErkmannWilliam F Marzluff
May 18, 2005·Trends in Biochemical Sciences·Roger D Kornberg
Jun 15, 2005·Trends in Cell Biology·Anthony H Futerman, Howard Riezman
Dec 1, 2005·Glycobiology·Daniel J Kelleher, Reid Gilmore
Dec 27, 2005·Experimental Cell Research·Judah Folkman
Jan 13, 2006·Trends in Cell Biology·Daniel UngarFrederick M Hughson
Feb 3, 2006·Molecular Biology of the Cell·Nicole R QuennevilleElizabeth Conibear
Feb 28, 2007·Nature Reviews. Microbiology·Stephen P Goff
Jul 17, 2007·Nature Reviews. Microbiology·Jan Balzarini
Jul 21, 2007·Science·Jacques FellayDavid B Goldstein
Aug 8, 2007·The Journal of Cell Biology·Nafiseh SabriChristos Samakovlis
Nov 17, 2007·Genes & Development·Noboru Mizushima

❮ Previous
Next ❯

Citations

Nov 22, 2011·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Matthew T Stier, Katherine R Spindler
Jan 16, 2010·Molecules and Cells·Donna Sir, Jing-hsiung James Ou
Feb 10, 2009·Apoptosis : an International Journal on Programmed Cell Death·M-L Gougeon, M Piacentini
Sep 23, 2009·Apoptosis : an International Journal on Programmed Cell Death·Manoj Kumar Tripathy, Debashis Mitra
Aug 10, 2011·Pharmaceutical Research·Priya S Shah, David V Schaffer
Mar 9, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Lisa J Henderson, Lena Al-Harthi
Mar 5, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Kate L JonesJohnson Mak
Jul 27, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Irena Kadiu, Howard E Gendelman
Apr 16, 2009·Current Infectious Disease Reports·Alexandra M Ortiz, Guido Silvestri
Aug 20, 2010·Immunologic Research·Marisa S Briones, Samson A Chow
Sep 4, 2012·Immunologic Research·Francesca Alvarez-CalderonJames DeGregori
Oct 3, 2012·Current Opinion in Virology·Benoît de ChasseyVincent Lotteau
Jul 6, 2013·Trends in Immunology·Manuele RebsamenGiulio Superti-Furga
Oct 15, 2013·Trends in Cell Biology·Raphaël GaudinPhilippe Benaroch
Jun 6, 2013·Trends in Microbiology·Joshua Tan, Quentin J Sattentau
Apr 15, 2014·Virology·Nicholas R MeyersonSara L Sawyer
Jan 12, 2011·Biochemistry·Andrew M L Lever, Kuan-Teh Jeang
Dec 15, 2010·ACS Chemical Biology·Frederic D Sigoillot, Randall W King
Jun 14, 2013·Journal of Medicinal Chemistry·Ya-Qiu LongNouri Neamati
Jan 4, 2013·Journal of Proteome Research·Cameron J SchweitzerMichael Belshan
Sep 6, 2008·Cell Death and Differentiation·A Orvedahl, B Levine
Jul 17, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Gregory A Barker, Scott L Diamond
Apr 25, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·John J Rossi
Apr 8, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yoon-Sang KimArthur W Nienhuis
Sep 29, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jiehua ZhouJohn J Rossi
Feb 10, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Alexis J WallenScott L Diamond
Nov 21, 2012·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jiehua ZhouJohn J Rossi
Dec 23, 2009·Nature·Renate KönigSumit K Chanda
Dec 23, 2011·Nature·Stefanie JägerNevan J Krogan
Sep 21, 2013·Nature·Caroline GoujonMichael H Malim
Oct 16, 2012·Nature Biotechnology·Xu TanStephen J Elledge
Mar 10, 2010·Nature Biotechnology·Ji-Young Min, Kanta Subbarao
Oct 11, 2008·Nature Biotechnology·Yueh-Ming Loo, Michael Gale
Jul 22, 2009·Nature Immunology·Sonia Sharma, Anjana Rao
Jul 26, 2008·Nature Reviews. Immunology·Ramnik J Xavier, John D Rioux
Jul 26, 2008·Nature Reviews. Immunology·Michael G KatzeMarcus J Korth
Oct 7, 2008·Nature Reviews. Immunology·Keiko OzatoHerbert C Morse

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