Identification of human gene products containing Pro-Pro-x-Tyr (PY) motifs that enhance glutathione and endocytotic marker uptake in yeast.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Shujie ShiN Ballatori

Abstract

In an attempt to identify genes involved in glutathione (GSH) transport, a human mammary gland cDNA library was screened for clones capable of complementing a defect in GSH uptake in yeast cells that lack Hgt1p, the primary yeast GSH uptake transporter. Five genes capable of rescuing growth on sulfur-deficient GSH-containing medium were identified: prostate transmembrane protein, androgen induced 1 (PMEPA1); lysosomal-associated protein transmembrane 4 alpha (LAPTM4alpha); solute carrier family 25, member 1 (SLC25A1); lipopolysaccharide-induced TNF factor (LITAF); and cysteine/tyrosine-rich-1 (CYYR1). All of these genes encode small integral membrane proteins of unknown function, although none appear to encode prototypical GSH transporters. Nevertheless, they all increased both intracellular glutathione levels and [(3)H]GSH uptake rates. [(3)H]GSH uptake was uniformly inhibited by high concentrations of unlabeled GSH, GSSG, and ophthalmic acid. Interestingly, each protein is predicted to contain Pro-Pro-x-Tyr (PY) motifs, which are thought to be important for regulating protein cell surface expression. Uptake of the endocytotic markers lucifer yellow and FM4-64 was also enhanced by each of the five genes. Mutations of the PY mo...Continue Reading

References

Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·H I Chen, M Sudol
Dec 4, 1998·Bioscience, Biotechnology, and Biochemistry·T MiyakeB Ono
Mar 13, 1999·The Journal of Clinical Investigation·H AbrielO Staub
Mar 18, 2000·Kidney International·O StaubD Rotin
May 2, 2000·The Journal of Biological Chemistry·A BourboulouxA K Bachhawat
Jun 7, 2002·The Journal of Biological Chemistry·Angelos-Aristeidis KonstasSharad Kumar
Feb 1, 2003·Gene·Nianshu ZhangStephen G Oliver
May 4, 2004·Annals of Neurology·Craig L BennettPhillip F Chance
Jun 16, 2004·International Journal of Cancer. Journal International Du Cancer·Yasuhiro MatsumuraYoshiki Miyachi
Apr 23, 2005·Toxicology and Applied Pharmacology·Nazzareno BallatoriRosemarie Marchan
Sep 9, 2006·Blood·Cinta Mestre-EscorihuelaJose A Martinez-Climent
Apr 6, 2007·Biomedizinische Technik. Biomedical Engineering·Bernd BaumannChristof P Rader
Aug 28, 2007·The International Journal of Biochemistry & Cell Biology·Sabyasachi BhattacharyaDale S Haines
Jan 30, 2008·Current Protocols in Cell Biology·S E Rieder, S D Emr
Sep 13, 2008·Molecular Aspects of Medicine·Nazzareno BallatoriChristine L Hammond
Jan 27, 2009·Biological Chemistry·Nazzareno BallatoriChristine L Hammond

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Citations

Jul 2, 2014·Molecular Biology Reports·Raffaella CasadeiFlavia Frabetti

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