Abstract
Since autoantibodies to IL-1alpha, interferon-alpha (IFN-alpha) and IL-6 have been described, this study concentrated on the search for autoantibodies to hIL-10 using an assay based on the precipitation of 125I-hIL-10 autoantibody complexes using Protein G-Sepharose. Among 1860 tested sera, only seven were found to specifically precipitate IL-10, thus indicating the rare occurrence of such autoantibodies. Four of those seven anti-IL-10 autoantibody sera were specific for hIL-10, two recognized both human and viral IL-10, while the last one recognized human, viral and murine IL-10, thus suggesting the existence of at least three different epitopic specificities. The purification of anti-IL-10 autoantibody from one serum demonstrated the existence of a single (IgG1, lambda) autoantibody that neutralized IL-10 biological activity. Thus, autoantibodies to IL-10 may represent natural antagonists to IL-10.
Citations
Aug 14, 1999·Immunology·A MeagerN Willcox
Jan 29, 2000·Immunology·M WadhwaR Thorpe
Jan 1, 1997·Biotherapy·P H van der Meide, H Schellekens
Dec 10, 1999·American Journal of Reproductive Immunology : AJRI·L SzeredayJ Szekeres-Bartho
May 10, 2008·Médecine sciences : M/S·Laure Coulombel, Armand Bensussan
Jan 11, 2007·Médecine sciences : M/S·Yves DelnestePascale Jeannin
Jul 28, 2009·Journal of Immunological Methods·Carina de Lemos RieperMorten Bagge Hansen
Feb 14, 2009·Cytokine & Growth Factor Reviews·Carina de Lemos RieperMorten Bagge Hansen
Mar 26, 2003·Clinical and Experimental Immunology·A MeagerN Willcox
Mar 14, 2007·Vox Sanguinis·M B HansenP Gimsing
Dec 17, 2008·Clinical and Experimental Immunology·E C EbertM Bajpai
Oct 4, 2007·Annals of the New York Academy of Sciences·Laëtitia Le PottierJacques-Olivier Pers
Nov 16, 2004·Journal of Immunological Methods·Ignacio AmadeoMarina Etcheverrigaray
Mar 16, 2011·The Journal of General Virology·Carina de Lemos RieperMorten Bagge Hansen
Jul 1, 2017·PloS One·Jakob Hjorth von StemannMorten Bagge Hansen
Jul 27, 1999·Circulation·S E EpsteinJ Zhu
Nov 29, 2008·Journal of Leukocyte Biology·Wolf-Dietrich DöckeHans-Dieter Volk
Dec 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Anne PuelJean-Laurent Casanova
Dec 16, 2019·Immunology Letters·Elena DonSergey Tarasov