Identification of human pituitary growth hormone variants by mass spectrometry

Journal of Proteome Research
Maxie KohlerMario Thevis

Abstract

The heterogeneity of human endogenous growth hormone (GH) is used in doping control analysis to distinguish it from the homogeneous recombinant analogue in plasma samples. Pituitary GH variants were characterized by gel electrophoresis and mass spectrometry. Besides 22 and 20 kDa isoforms, fragments of 9 and 12 kDa were identified and a glycosylated 23 kDa GH variant was elucidated to bear a HexHexNac 2 NeuAc modification presumably located at Thr 60.

References

Oct 15, 1992·European Journal of Biochemistry·A Kobata
Dec 1, 1992·Clinical Pediatrics·V I RickertM S Jay
Apr 30, 1985·Biochemical and Biophysical Research Communications·J P LibertiJ F Chlebowski
Jan 29, 1980·Biochemical and Biophysical Research Communications·U J LewisL J Lewis
Nov 12, 1996·Biochemical and Biophysical Research Communications·L S HaroN C Ling
Aug 31, 2001·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·P J Jenkins
Mar 1, 2003·Nature Biotechnology·Matthias Mann, Ole N Jensen
Jun 12, 2003·Sports Medicine·Richard J GodfreyGregory P Whyte
Mar 5, 2004·Proteomics·Francesco GiorgianniDominic M Desiderio
Feb 18, 2005·Proteomics·Xianquan ZhanDominic M Desiderio
Sep 13, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Elio F De PaloPaolo Spinella
Jun 30, 2006·Pituitary·Sarka Beranova-GiorgianniFrancesco Giorgianni
Nov 2, 2006·Pituitary·M DogaA Giustina
Dec 15, 2006·The New England Journal of Medicine·Shlomo Melmed
Jun 24, 2008·Growth Factors·Gerard Such-SanmartínRicardo Gutiérrez-Gallego
Nov 13, 2008·Electrophoresis·Maxie KohlerMario Thevis

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Citations

Mar 31, 2011·Analytical and Bioanalytical Chemistry·R Gutiérrez-GallegoJ Segura
May 13, 2011·Analytical and Bioanalytical Chemistry·Lindsey G Mackay, Rymantas Kazlauskas
Aug 9, 2013·Analytical and Bioanalytical Chemistry·Mario ThevisWilhelm Schänzer
Aug 1, 2009·Bioanalysis·Chunji He, Moutian Wu
Aug 28, 2014·Bioanalysis·Francesco GiorgianniSarka Beranova-Giorgianni
Jul 16, 2009·Growth Factors·Gerard Such-SanmartínRicardo Gutiérrez-Gallego
Aug 25, 2011·Forensic Science International : Synergy·Christian Reichel
Aug 3, 2010·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Maxie KohlerMario Thevis
Feb 4, 2010·Endocrinology and Metabolism Clinics of North America·Martin Bidlingmaier, Jenny Manolopoulou
Sep 1, 2011·Mass Spectrometry Reviews·Peihong ZhuJohn G Marshall
Apr 9, 2010·Drug Testing and Analysis·Mario ThevisWilhelm Schänzer
Jan 4, 2015·Biochimica Et Biophysica Acta·Gerard Such-SanmartínOle N Jensen
Mar 17, 2010·Analytical Biochemistry·Cristian G ArseneMartin Bidlingmaier
Jun 16, 2016·Frontiers in Endocrinology·Xianquan ZhanTingting Cheng
Apr 17, 2018·Endocrinology·Shutan LiaoJo K Perry
Jun 18, 2017·The EPMA Journal·Tingting Cheng, Xianquan Zhan

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