Identification of human testis-specific transcripts and analysis of their expression in tumor cells

Biochemical and Biophysical Research Communications
C De SmetT Boon

Abstract

Tumor-specific antigens recognized by autologous T lymphocytes are encoded by genes, including those of the MAGE, BAGE, and GAGE gene families, that are expressed in a significant fraction of tumors of various types, but not in normal adult tissues, except for testis where they appear to be expressed in germ cells. Because male germ cells are known to express many genes that are not expressed in other normal adult tissues, we wished to determine whether most of these genes are occasionally activated in tumor cells. Representational difference analysis was used to obtain testis-specific transcripts. The expression of 15 testis-specific cDNA sequences was tested by RT-PCR in a series of tumor cell lines. Only one cDNA sequence showed a significant level of expression in some tumor cell lines. Remarkably, this cDNA clone proved to be a new gene of the MAGE family. These results suggest that MAGE, BAGE, and GAGE genes belong to a minor subset of testis-specific genes that is often activated in tumors of various types, whereas most testis-specific genes are either never or very rarely activated in tumors.

References

Nov 1, 1992·Developmental Biology·J R McCarreyJ J Rossi
Nov 11, 1992·International Journal of Cancer. Journal International Du Cancer·F BrasseurT Boon
Jan 1, 1992·Mammalian Genome : Official Journal of the International Mammalian Genome Society·N MizukiM Kasahara
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M KasaharaJ Klein
Jan 1, 1986·Molecular and Cellular Biology·C Mann, R W Davis
Oct 11, 1983·Nucleic Acids Research·M A Gama-SosaM Ehrlich
Nov 15, 1995·International Journal of Cancer. Journal International Du Cancer·H InoueT Akiyoshi
Jan 1, 1995·Biology of Reproduction·K D FulcherE M Eddy
Mar 1, 1994·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·J del MazoM Ferraro
Mar 15, 1994·International Journal of Cancer. Journal International Du Cancer·P WeynantsT Boon
Jan 1, 1994·Immunogenetics·C De SmetT Boon
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·U SahinM Pfreundschuh
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·L DiatchenkoP D Siebert
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·C De SmetT Boon
Nov 1, 1996·Human Molecular Genetics·F SchniedersJ Schmidtke
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y T ChenL J Old
Apr 1, 1997·Mammalian Genome : Official Journal of the International Mammalian Genome Society·C NiederbergerC E Bishop
Mar 1, 1997·Genomics·E De PlaenT Boon

❮ Previous
Next ❯

Citations

Aug 5, 1999·Kidney International·H ZhangH Makino
Nov 26, 2002·Immunological Reviews·Pierre Van Der BruggenThierry Boon
Mar 21, 2002·Cancer Investigation·Alexei F KirkinJesper Zeuthen
May 4, 2004·Cancer Genetics and Cytogenetics·Xiaoming YaoAndrew R Hoffman
Sep 18, 1998·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·A F KirkinJ Zeuthen
Apr 22, 2003·International Journal of Cancer. Journal International Du Cancer·Axelle LoriotCharles De Smet
Jun 28, 2002·British Journal of Cancer·M TakimotoN Kuzumaki
Dec 23, 2004·Journal of Immunotherapy·Yuriko MatsuzakiYutaka Kawakami
Aug 16, 2012·Molecular and Cellular Biochemistry·G S Gupta
May 12, 2006·Molecular Cancer Research : MCR·Frank WischnewskiHeidi Schwarzenbach

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.