Identification of hyaluronidase and phospholipase B in Lachesis muta rhombeata venom

Toxicon : Official Journal of the International Society on Toxinology
Gisele A WiezelEliane C Arantes

Abstract

Hyaluronidases contribute to local and systemic damages after envenoming, since they act as spreading factors cleaving the hyaluronan presents in the connective tissues of the victim, facilitating the diffusion of venom components. Although hyaluronidases are ubiquitous in snake venoms, they still have not been detected in transcriptomic analysis of the Lachesis venom gland and neither in the proteome of its venom performed previously. This work purified a hyaluronidase from Lachesis muta rhombeata venom whose molecular mass was estimated by SDS-PAGE to be 60 kDa. The hyaluronidase was more active at pH 6 and 37 °C when salt concentration was kept constant and more active in the presence of 0.15 M monovalent ions when the pH was kept at 6. Venom was fractionated by reversed-phase liquid chromatography (RPLC). Edman sequencing after RPLC failed to detect hyaluronidase, but identified a new serine proteinase isoform. The hyaluronidase was identified by mass spectrometry analysis of the protein bands in SDS-PAGE. Additionally, phospholipase B was identified for the first time in Lachesis genus venom. The discovery of new bioactive molecules might contribute to the design of novel drugs and biotechnology products as well as to deve...Continue Reading

References

Aug 1, 1992·Toxicon : Official Journal of the International Society on Toxinology·M A CevallosA C Alagon
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·S PukrittayakameeD Bunnag
Jan 1, 1987·Toxicon : Official Journal of the International Society on Toxinology·A W BernheimerK S Kim
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·A W BernheimerR Linder
Sep 1, 1964·The Biochemical Journal·H M Doery, J E Pearson
Mar 1, 1967·European Journal of Biochemistry·P Edman, G Begg
Jan 1, 1983·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A T Tu, R R Hendon
May 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·A S AguiarS Giovanni-de-Simone
Apr 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·M T JorgeD A Warrell
Dec 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·R OteroJ M Gutiérrez
Mar 10, 2000·Biochimica Et Biophysica Acta·T MatsuiK Titani
Aug 2, 2001·Toxicon : Official Journal of the International Society on Toxinology·A C PessiniE C Arantes
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·R Manjunatha Kini
Mar 17, 2004·Toxicon : Official Journal of the International Society on Toxinology·Bruno LomonteLeonel Calderón
Aug 3, 2004·The Biochemical Journal·Shuting LiSiqi Liu
Jun 23, 2005·Toxicon : Official Journal of the International Society on Toxinology·Daniela C S DamicoJosé Camillo Novello
Aug 17, 2005·Journal of Thrombosis and Haemostasis : JTH·Q LuK J Clemetson
Jun 6, 2007·Toxicon : Official Journal of the International Society on Toxinology·Shivaiah NagarajuKempaiah Kemparaju
Feb 28, 2008·Journal of Mass Spectrometry : JMS·P EscoubasG M Nicholson
Dec 10, 2008·Rapid Communications in Mass Spectrometry : RCM·William R AlleyMilos V Novotny
Jan 30, 2010·Lancet·David A Warrell
Jan 13, 2011·Toxicon : Official Journal of the International Society on Toxinology·Karinne Cristinne da Silva CunhaElizabeth Giestal de Araujo
Aug 2, 2011·Journal of Proteomics·Paula H C CiscottoAdriano M C Pimenta
Apr 7, 2012·Journal of Proteomics·Renata S RodriguesVeridiana M Rodrigues
Sep 12, 2012·Toxicon : Official Journal of the International Society on Toxinology·Cristiane Bregge-SilvaEliane Candiani Arantes
Sep 25, 2012·Toxicon : Official Journal of the International Society on Toxinology·A F WahbyAfaf S Fahmy
Dec 12, 2012·Cell Reports·Xiomaris M Cotto-RiosTony T Huang
Apr 13, 2013·Toxicon : Official Journal of the International Society on Toxinology·Juan J Calvete
Dec 4, 2013·Proceedings of the National Academy of Sciences of the United States of America·Freek J VonkMichael K Richardson
Feb 20, 2014·PLoS Neglected Tropical Diseases·Carolina Campolina Rebello HortaEvanguedes Kalapothakis
Oct 29, 2014·Nucleic Acids Research·UNKNOWN UniProt Consortium

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Citations

Jul 5, 2016·Journal of Proteomics·Carolina A NicolauRichard H Valente
Dec 26, 2016·Biochimica Et Biophysica Acta. General Subjects·Johara Boldrini-FrançaEliane Candiani Arantes
Feb 20, 2018·Applied Microbiology and Biotechnology·Fernanda Gobbi AmorimEliane Candiani Arantes
Apr 17, 2018·PLoS Neglected Tropical Diseases·Stephanie StranskyVania Maria Martin Braga
Dec 1, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Francielle Almeida CordeiroEliane Candiani Arantes
May 28, 2019·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Gisele A WiezelEliane C Arantes
Aug 28, 2020·Frontiers in Molecular Biosciences·Anwar Ullah, Rehana Masood
Dec 17, 2019·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Prapenpuksiri RungsaSakda Daduang
Sep 5, 2018·Toxicon : Official Journal of the International Society on Toxinology·Mônika Aparecida CoronadoRaghuvir Krishnaswamy Arni
Mar 17, 2020·Toxicon : Official Journal of the International Society on Toxinology·Syeda U HashmiSyed A Ali

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