Identification of hypoxia-responsive genes in a dopaminergic cell line by subtractive cDNA libraries and microarray analysis

Parkinsonism & Related Disorders
D Beitner-JohnsonDavid E Millhorn

Abstract

Transplantation of dopamine-secreting cells harvested from fetal mesencephalon directly into the striatum has had limited success as a therapy for Parkinson's disease. A major problem is that the majority of the cells die during the first 3 weeks following transplantation. Hypoxia in the tissue surrounding the graft is a potential cause of the cell death. We have used subtractive cDNA libraries and microarray analysis to identify the gene expression profile that regulates tolerance to hypoxia. An improved understanding of the molecular basis of hypoxia-tolerance may allow investigators to engineer cells that can survive in the hypoxic environment of the brain parenchyma following transplantation.

References

Apr 1, 1992·Journal of Neurochemistry·M F Czyzyk-KrzeskaD E Millhorn
Jan 1, 1990·Annual Review of Neuroscience·D M Yurek, J R Sladek
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·F Antequera, A Bird
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·G L WangG L Semenza
Oct 6, 1995·The Journal of Biological Chemistry·M L Norris, D E Millhorn
Jun 2, 1995·The Journal of Biological Chemistry·A P LevyM A Goldberg
Jan 20, 1995·The Journal of Biological Chemistry·G L Wang, G L Semenza
Sep 1, 1996·Molecular and Cellular Biology·J A ForsytheG L Semenza
Aug 1, 1996·The American Journal of Physiology·W H ZhuD E Millhorn
Feb 12, 1998·Science·L RowenL Hood
May 20, 1998·BioTechniques·C LockeyZ Long
Jul 25, 1998·The Journal of Biological Chemistry·D Beitner-Johnson, D E Millhorn
Sep 5, 1998·Brain Research. Molecular Brain Research·R R MishraN R Prabhakar
Jan 23, 1999·BioTechniques·W M FreemanK E Vrana
Jan 23, 1999·Nature Genetics·P O Brown, D Botstein
Aug 7, 1999·The Journal of Biological Chemistry·P W ConradD Beitner-Johnson
Sep 11, 1999·Annals of Neurology·L W WhitneyW E Biddison
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·M WilsonG K Schoolnik
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·G JohannesP Sarnow
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·J KhanP S Meltzer
Nov 24, 1999·The Journal of Biological Chemistry·P W ConradD E Millhorn
Dec 11, 1999·Pflügers Archiv : European journal of physiology·S KobayashiD E Millhorn
May 2, 2000·The Journal of Physiology·L ConfortiD E Millhorn
Jun 13, 2000·Advances in Experimental Medicine and Biology·D E MillhornR Rust
Aug 6, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·F MagdinierR Dante
Feb 28, 2002·Science's STKE : Signal Transduction Knowledge Environment·C S Hill, R Treisman

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Citations

Mar 18, 2003·International Journal of Cancer. Journal International Du Cancer·Joerg SchlingemannPeter Lichter
Jan 22, 2002·Metabolic Engineering·David J OliverEve Syrkin Wurtele
Dec 19, 2003·Biochemical and Biophysical Research Communications·Changjiang LiuDaiming Fan
Jan 13, 2004·Journal of Applied Physiology·Karen A Seta, David E Millhorn

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