Identification of immune biomarkers related to disease progression and treatment efficacy in human visceral leishmaniasis

Immunobiology
Áquila S B PortelaEduardo Antonio Ferraz Coelho

Abstract

Visceral leishmaniasis (VL) is a potentially fatal disease, in which the treatment based on chemotherapy is considered toxic. The cure of disease is associated with the life-long Th1-type immunity against the infection. The Th1-related cytokines production by peripheral blood mononuclear cells (PBMCs) seems to be crucial for host control of parasite load and clinical cure. In the current study, we used five proteins (IgE-dependent histamine-releasing factor [HRF], LiHyD, LiHyV, LiHyT and LiHyp6) recently shown to be antigenic and/or immunogenic in the canine VL, aiming to evaluate the antigen-specific antibody levels and cytokine production in PBMCs culture supernatants collected from VL patients before and after anti-VL treatment. In the results, when PBMCs were exposed to rHRF, rLiHyD and rLiHyT, higher IFN-γ and lower IL-10 levels were observed in all patients that were treated and clinically cured. Analysis of specific antibody subclasses was in line with in vitro cellular response, since a higher IgG2 production was found in the treated and cured patients, when compared to the IgG1 subclass levels. In addition, evaluating the diagnostic efficacy of the recombinant molecules, the rHRF, rLiHyD and rLiHyT proteins showed the ...Continue Reading

Citations

Mar 12, 2020·Frontiers in Cellular and Infection Microbiology·Maude F LévêqueChristelle Pomares
Nov 1, 2020·Cytokine·Renuka BhorKalpana Pai
Mar 12, 2021·Frontiers in Cellular and Infection Microbiology·Srija MoulikMitali Chatterjee

❮ Previous
Next ❯

Related Concepts