Identification of Immune Cell Infiltration and Immune-Related Genes in the Tumor Microenvironment of Glioblastomas.

Frontiers in Immunology
Sicong HuangJianwei Pan

Abstract

Glioblastoma (GBM) is one of the most prevalent malignant brain tumors with poor prognosis. Increasing evidence has revealed that infiltrating immune cells and other stromal components in the tumor microenvironment (TME) are associated with prognosis of GBM. The aim of the present study was to identify immune cells and immune-related genes extracted from TME in GBM. RNA-sequencing and clinical data of GBM were downloaded from The Cancer Genome Atlas (TCGA). Four survival-related immune cells were identified via Kaplan-Meier survival analysis and immune-related differentially expressed genes (DEGs) screened. Functional enrichment and protein-protein interaction (PPI) networks for the genes were constructed. In addition, we identified 24 hub genes and the expressions of 6 of the genes were significantly associated with prognosis of GBM. Finally, the genes were validated in single-cell sequencing studies of GBM, and the immune cells validated in an independent GBM cohort from the Chinese Glioma Genome Atlas (CGGA). Overall, 24 immune-related genes infiltrating the tumor microenvironment were identified in the present study, which could serve as novel biomarkers and immune therapeutic targets.

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Citations

Sep 21, 2021·Frontiers in Immunology·Saadia Ait SsiAbdallah Badou

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Methods Mentioned

BETA
surgical resection
single-cell sequencing
RNA-Seq
Feature Plot
single-
cell sequencing
conventional
ScRNA-seq

Software Mentioned

Seurat R
edgeR R package
Search Tool for the Retrieval of
R
Cytoscape
STRING
Feature Plot
DAVID
TIMER
CIBERSORT

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