PMID: 7543117May 1, 1995Paper

Identification of immunodominant epitopes in the core and non-structural region of hepatitis C virus by enzyme immunoassay using synthetic peptides

Journal of Immunoassay
H J ParkH M Moon

Abstract

Thirty-two synthetic peptides, components of the core and non-structural protein of Hepatitis C virus (HCV), were tested for their reactivities against antibodies in sera of healthy, HCV antibody positive of chronic liver disease patients. Among them, 8 of the core peptides, 4 of the NS4 peptides and 3 of the NS5 peptides reacted with the HCV infected sera. In particular, C22 (core peptide) and NS4-1924 (NS4 peptide) were most reactive with the serum samples giving a positive signal with commercially available enzyme-linked immunosorbent assay (ELISA) kit. Our results indicate that the immunodominant regions of the HCV-derived proteins are located at three regions in the core protein, three regions in the NS4 protein, and one region in the NS5 protein. These results indicate that the selected peptides are useful antigens in detecting antibodies in the sera from individuals infected with HCV.

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W M ChingJ W Shih
Jun 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·M S NasoffA M Prince
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N KatoK Shimotohno
Sep 2, 1985·FEBS Letters·G W WellingS Welling-Wester
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Jan 1, 1980·Methods in Enzymology·E Engvall

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Citations

Aug 23, 2001·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·H TamimW Y Almawi
Nov 5, 1997·The Veterinary Quarterly·R H MeloenJ P Langeveld
Dec 1, 2015·Journal of Virological Methods·Teppo SalminenSheikh M Talha
Oct 13, 2009·Biophysical Chemistry·Yeng-Tseng WangCheng-Lung Chen
Nov 13, 2001·Clinical and Laboratory Haematology·N Irani-HakimeW Y Almawi
Oct 14, 2003·FEMS Microbiology Letters·Su-Min KangJong-Won Oh
Feb 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Renée MénezEnrico A Stura

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