Identification of immunodominant proteins of the microalgae Prototheca by proteomic analysis

New Microbes and New Infections
A IrrgangUwe Roesler

Abstract

Prototheca zopfii associated with bovine mastitis and human protothecosis exists as two genotypes, of which genotype 1 is considered as non-infectious and genotype 2 as infectious. The mechanism of infection has not yet been described. The present study was aimed to identify genotype 2-specific immunodominant proteins. Prototheca proteins were separated using two-dimensional gel electrophoresis. Subsequent western blotting with rabbit hyperimmune serum revealed 28 protein spots. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis resulted in the identification of 15 proteins including malate dehydrogenase, elongation factor 1-alpha, heat shock protein 70, and 14-3-3 protein, which were previously described as immunogenic proteins of other eukaryotic pathogens.

References

Dec 13, 2006·Veterinary Microbiology·Asja MöllerUwe Roesler
Aug 24, 2007·Applied Microbiology and Biotechnology·Matthias BerthJörg Bernhardt
Apr 25, 2008·Journal of Clinical Microbiology·Sara MarquesGertrude Thompson
Mar 21, 2009·Medical Mycology·Gertrude ThompsonJúlio Carvalheira
Aug 12, 2009·International Journal of Systematic and Evolutionary Microbiology·Kazuo SatohKoichi Makimura
Nov 17, 2009·The Journal of Veterinary Medical Science·Yuji KishimotoHiroshi Kamata
Jul 1, 2009·Proteomics. Clinical Applications·Martin von BergenUwe Roesler
Jul 26, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J MurugaiyanU Roesler
Mar 22, 2014·PLoS Neglected Tropical Diseases·Fernanda LudolfRosiane A Silva-Pereira

❮ Previous
Next ❯

Citations

Dec 31, 2016·International Journal of Molecular Sciences·Jayaseelan MurugaiyanUwe Roesler
May 17, 2018·Medical Mycology·John R ToddR Scott Pore
Aug 30, 2019·Journal of Proteomics·Carlos FajardoFrancisco Javier Fernandez-Acero

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

Decodon
Prototheca
MASCOT
Delta2D

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.