PMID: 7581341Sep 1, 1995Paper

Identification of immunogenic epitopes of the 170-kDa subunit adhesin of Entamoeba histolytica in patients with invasive amebiasis

The Journal of Eukaryotic Microbiology
C VelazquezJ Calderon

Abstract

Entamoeba histolytica causes amebic dysentery (AD) and liver abscess (ALA). Little is known about protective immunity to amebiasis, and studies in this area have been complicated by the paucity of defined ameba antigens. We examined the proliferative responses of peripheral blood mononuclear cells (PBMC) from patients with AD and ALA to a recombinant protein containing a portion of the 170 kDa adhesin of E. histolytica (170CR), and to two synthetic peptides (1 and 2) derived from the 170 kDa sequence that were predicted to contain T cell epitopes. A significant number of patients with AD and ALA had PBMC that proliferated to 170CR molecule, and several individuals with ALA and AD had T cells that recognized one or both peptides. Contrarily, individuals from a non-endemic region for amebiasis did not respond to 170CR protein, or to both peptides. In regard to antibody response, nine of fifteen patients with ALA showed antibodies to 170CR protein. These same patients had antibodies to peptide 2. We identified peptides from 170-kDa adhesin that may contain both T and B cell epitopes recognized by some patients with invasive amebiasis. These peptides may be valuable reagents in studies of the immune response to amebiasis.

References

Mar 1, 1992·European Journal of Immunology·Y ZeveringM F Good
Jan 1, 1990·Current Topics in Microbiology and Immunology·J B Rothbard, J R Lamb
Mar 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E TannichR D Horstmann
Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B J MannW A Petri
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·C DeLisi, J A Berzofsky
Mar 1, 1986·Reviews of Infectious Diseases·R L Guerrant
Jan 1, 1970·The American Journal of Tropical Medicine and Hygiene·I M Krupp
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
Mar 1, 1983·Infection·F KeesL Safrany
Dec 1, 1983·Infection and Immunity·E GhadirianP A Kongshavn
Nov 1, 1993·Molecular and Biochemical Parasitology·J E PurdyW A Petri
Sep 1, 1994·Journal of Endovascular Surgery : the Official Journal of the International Society for Endovascular Surgery·N R Hertzer

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