Identification of immunogenic outer membrane proteins of Haemophilus influenzae type b in the infant rat model system.

Infection and Immunity
E J HansenK H Johnston

Abstract

Outer membrane proteins of Haemophilus influenzae type b which are immunogenic in infant rats were identified by a radioimmunoprecipitation method. Intact cells of H. influenzae type b were radioiodinated by a lactoperoxidase-catalyzed procedure, and an outer membrane-containing fraction was prepared from these cells. These radioiodinated outer membranes were mixed with sera obtained from rats convalescing from systemic H. influenzae type b disease induced at 6 days of age, and the resultant (antibody-outer membrane protein antigen) complexes were extracted from these membranes by treatment with nonionic detergent and ethylenediaminetetraacetic acid. These soluble antibody-antigen complexes were isolated by means of adsorption to protein A-bearing staphylococci, and the radioiodinated protein antigens were identified by gel electrophoresis followed by autoradiography. Infant rats were shown to mount a readily detectable antibody response to several different proteins present in the outer membrane of H. influenzae type b. Individual infant rats were found to vary both qualitatively and quantitatively in their immune response to these immunogenic outer membrane proteins.

References

Jan 1, 1977·The Journal of Infectious Diseases·P F WellerD H Smith
Oct 1, 1978·The Journal of Infectious Diseases·P F WellerP Anderson
Jul 1, 1973·Journal of Bacteriology·H G RittenhouseJ Heptinstall
Jan 1, 1972·The Journal of Clinical Investigation·P AndersonD H Smith
Jan 1, 1972·Proceedings of the Society for Experimental Biology and Medicine·C W Norden
Mar 1, 1980·Journal of Bacteriology·R L McDade, K H Johnston
Mar 1, 1980·The Journal of Experimental Medicine·J B Baseman, E C Hayes
Mar 1, 1980·Infection and Immunity·C E MachamerH J Zweerink
Jan 31, 1932·The Journal of Experimental Medicine·J Wright, H K Ward

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Citations

Sep 1, 1989·American Journal of Respiratory Cell and Molecular Biology·J L McGeheeE J Hansen
Aug 1, 1982·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section C, Immunology·T Dahlberg-Lagergård
Apr 1, 1993·Clinical Microbiology Reviews·A R Tunkel, W M Scheld
Dec 1, 1987·Infection and Immunity·P A Gulig, R Curtiss
Nov 1, 1989·Research in Microbiology·K BrzostekW Boos
Jul 1, 1983·Infection and Immunity·J P BuransM Solotorovsky
Jul 1, 1985·Infection and Immunity·C V SciortinoR A Finkelstein
Mar 1, 1984·Infection and Immunity·M J BlaserM L Vasil
Jan 1, 1984·Infection and Immunity·R C HedstromD R Galloway
Dec 1, 1990·Journal of Clinical Microbiology·J MertsolaE J Hansen
Mar 1, 1983·Infection and Immunity·J F Alderete
Jan 1, 1988·Infection and Immunity·E J HansenG B Toews

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