Mar 1, 1976

Identification of immunoglobulins associated with complement fixation, agglutination, and low pH buffered antigen tests for brucellosis

American Journal of Veterinary Research
J M PattersonS S Stone

Abstract

Serums from infected cattle, cattle with persistent postvaccinal antibody, and serologically "positive" noninfected cattle were fractionated into major immunoglobulin classes by diethylaminoethyl (DEAE)-cellulose chromatography and by sucrose density gradient centrifugation. Each fraction was assayed for anti-Brucella activity by standard tube-agglutination test (STT), buffered tube-agglutination test (BTT), and complement-fixation test (CF). In the serums from experimentally infected cattle, anti-Brucella antibody could be found by all tests in 6 DEAE fractions and in slow, fast, and sediment regions of the density gradient. Serums from cattle with persistent postvaccinal titers had STT activity in all 6 DEAE fractions, BTT activity in 5 fractions, and CF activity in only 1 fraction. The STT and BTT activities were found in the slow and the sediment regions of the gradient, whereas the CF activity was found only in the slow region. Serums from a chronically infected animal had STT and BTT activities in 2 DEAE fractions and CF activity in only 1. The STT, BTT, and CF activities were found in the slow and the sediment regions of the gradient. The principal antibody in serums from noninfected cattle was immunoglobulin M, which ha...Continue Reading

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Mentioned in this Paper

Immunoglobulins
Brucella Abortus Infection
Antigens, Bacterial
IgM2
Testicular Neoplasms
Polyglobin
Bos indicus
Hydrogen-Ion Concentration
Complement Fixation Tests
Agglutination Tests

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