Identification of immunoreactive plasma and stomach ghrelin, and expression of stomach ghrelin mRNA in the bullfrog, Rana catesbeiana

General and Comparative Endocrinology
Hiroyuki KaiyaS Kikuyama

Abstract

In this study, we established a radioimmunoassay (RIA) specific for ghrelin from the bullfrog Rana catesbeiana using a novel antibody raised against the C-terminal amino acid sequence of bullfrog ghrelin [13-28]. We also examined the distribution of ghrelin-producing cells in the stomachs of bullfrogs using this antibody and a cRNA probe specific for the bullfrog ghrelin gene. Ghrelin levels in plasma and stomach extracts were approximately 150 fmol/ml and 83-135 fmol/mg wet tissue, respectively. Reverse-phase high performance liquid chromatographic analysis, combined with bullfrog ghrelin RIA, revealed that ghrelin immunoreactivity in the stomach was composed of non-acylated ghrelin (des-acyl ghrelin) and several acylated forms of ghrelin bearing different fatty acid modifications, which could induce increases in intracellular Ca2+ in cells expressing the rat GH secretagogue receptor. In the stomach, the major storage form was acylated ghrelin. In bullfrog plasma, however, the majority of ghrelin immunoreactivity was des-acyl ghrelin and C-terminal fragments of frog ghrelin. Acylated ghrelin forms comprised only minor peaks. Ghrelin-immunopositive and ghrelin mRNA-expressing cells were observed within the mucosal layer of the ...Continue Reading

References

Nov 1, 1988·Scandinavian Journal of Gastroenterology·M SimonssonF Sundler
Feb 13, 2001·Biochemical and Biophysical Research Communications·H HosodaK Kangawa
Mar 13, 2001·Biochemical and Biophysical Research Communications·K ToshinaiS Matsukura
Jun 1, 2001·Regulatory Peptides·C Dornonville de la CourR Håkanson
Sep 8, 2001·The Journal of Biological Chemistry·H KaiyaK Kangawa
Oct 25, 2002·Journal of the American Society of Nephrology : JASN·Akihiro YoshimotoKazuwa Nakao
Jan 18, 2003·The Journal of Biological Chemistry·Nicholas J BeaumontKaila S Srai
May 7, 2003·Biochemical and Biophysical Research Communications·Ishwar S ParharYasuo Sakuma
Apr 15, 2004·European Journal of Endocrinology·Takashi AkamizuKenji Kangawa
Nov 24, 2004·Biochemical and Biophysical Research Communications·Yuka ShanadoNaoki Teno
Dec 29, 2004·Regulatory Peptides·Haruaki KageyamaSeiji Shioda
Mar 8, 2005·General and Comparative Endocrinology·Saad ShoushaNoboru Murakami
Mar 25, 2005·Physiological Reviews·Masayasu Kojima, Kenji Kangawa

❮ Previous
Next ❯

Citations

Aug 28, 2010·International Journal of Peptides·Ichiro Sakata, Takafumi Sakai
Jan 29, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hiroyuki KaiyaSuraj Unniappan
Jun 29, 2007·General and Comparative Endocrinology·Genciana TerovaMarco Saroglia
Jul 15, 2015·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Hiroyuki KaiyaMikiya Miyazato
Nov 12, 2013·Peptides·Shunsuke ShimizuKouhei Matsuda
Aug 16, 2011·Peptides·Yoshihiro NishiMasayasu Kojima
May 24, 2011·Peptides·Hiroyuki KaiyaKenji Kangawa
Jun 28, 2017·Endocrine Journal·Hiroyuki KaiyaMikiya Miyazato
Jun 4, 2019·Frontiers in Endocrinology·Takio Kitazawa, Hiroyuki Kaiya

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2022 Meta ULC. All rights reserved