Identification of Inflammatory Mediators in Tendinopathy Using a Murine Subacromial Impingement Model

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Claire D EliasbergScott A Rodeo

Abstract

Subacromial impingement is associated with a spectrum of disorders-including rotator cuff disease-but their relationship is complex. We have established a novel murine model of subacromial impingement to study supraspinatus tendinopathy. The purpose of this study was to evaluate changes in gene expression in this murine shoulder impingement model to further elucidate the mechanisms underlying the development of tendinopathy. Twenty-eight C57BL/6 mice were used in this study. All mice underwent bilateral surgery with insertion of a small metal clip in the subacromial space or a sham procedure. The supraspinatus tendons underwent histological analyses, biomechanical testing, and RNA extraction for multiplex gene expression analysis (NanoString, Seattle, WA). Histology demonstrated increased cellularity and disorganized collagen fibers of the supraspinatus tendon in the clip impingement group. Mean load to failure (5.20 vs. 1.50 N, p < 0.001) and mean stiffness (4.95 vs. 1.47 N/mm, p < 0.001) were lower in the impingement group than the sham group. NanoString analyses revealed 111 differentially expressed genes (DEGs) between the impingement and sham groups. DEGs of interest included Mmp3 (expression ratio [ER]: 2.68, p = 0.002), ...Continue Reading

References

Dec 1, 1995·Annals of the Rheumatic Diseases·D A van der WindtL M Bouter
Sep 30, 1998·Journal of Shoulder and Elbow Surgery·A G SchneebergerC Gerber
Oct 18, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ho-Rim ChoiHisashi Iwata
Sep 21, 2005·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·Ilya VoloshinTheodore A Blaine
Apr 3, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michael JosephAugustus D Mazzocca
Oct 3, 2009·Journal of Cell Communication and Signaling·Susan Amanda LundMarta Scatena
Apr 7, 2010·Human Genomics·Brian C JacksonVasilis Vasiliou
Nov 5, 2011·The Journal of the American Academy of Orthopaedic Surgeons·Alicia K Harrison, Evan L Flatow
Oct 10, 2013·Advances in Experimental Medicine and Biology·Ingo Schmitz
Mar 21, 2015·Cell Adhesion & Migration·Francesca ChiovaroMatthias Chiquet
Apr 14, 2015·Journal of Shoulder and Elbow Surgery·Mark S HsiaoBrett D Owens
Apr 24, 2018·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Guang-Ting CongScott A Rodeo

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Citations

Sep 17, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hongbin LuJianzhong Hu
Sep 18, 2020·The Journal of Bone and Joint Surgery. American Volume·Xueying ZhangScott A Rodeo
Jun 3, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Yunwei XiaLionel B Ivashkiv
Aug 10, 2021·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Farrah A MonibiScott A Rodeo

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