Identification of insulin-like growth factor binding proteins from cultured human epidermal keratinocytes

Journal of Cellular Physiology
M M MurashitaG A Werther

Abstract

The role and mechanisms of action of insulin-like growth factors (IGFs) in skin remain unclear. Epidermal keratinocytes possess IGF-I receptors and are responsive to IGF-I, which is primarily derived from underlying dermal fibroblasts. IGF binding proteins (IGFBPs), also synthesized by fibroblasts, may be involved in paracrine targeting of IGF-I to its receptors. We therefore examined whether human keratinocytes synthesize IGFBPs and their mRNAs. Following culture in complete medium (containing bovine pituitary extract and epidermal growth factor) Western ligand blotting (WLB) of cell conditioned medium revealed a major band of 32 kD, a less abundant IGFBP of 24 kD at all passages, and a 37-42 kD IGFBP which increased in abundance in late passage. Immunoprecipitation followed by WLB confirmed that the predominant 32 kD band was IGFBP-2. Radioimmunoassay of IGFBP-1, -3, and -6 revealed detectable levels of IGFBP-3 and significant levels of IGFBP-6, but not IGFBP-1. Northern analysis following culture in complete medium revealed that at early passage IGFBP-1, -2, -4, and -6 mRNAs were detectable. IGFBP-3 and -5 mRNAs were not detectable. Following culture in growth factor-free medium a 37-42 kD band, consistent with IGFBP-3, was ...Continue Reading

References

Feb 1, 1990·The Journal of Clinical Endocrinology and Metabolism·R G RosenfeldL C Giudice
Jan 1, 1991·The Journal of Investigative Dermatology·E K NeelyR G Rosenfeld
Dec 1, 1986·The Journal of Clinical Investigation·R C Baxter, J L Martin
Sep 1, 1987·The Journal of Clinical Endocrinology and Metabolism·R C BaxterM H Wood
Jan 1, 1993·Annual Review of Physiology·W S Cohick, D R Clemmons

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Citations

May 14, 1999·The Journal of Clinical Endocrinology and Metabolism·S R EdmondsonG A Werther
Dec 13, 2003·Endocrine Reviews·Stephanie R EdmondsonChristopher J Wraight
Sep 13, 2003·The Journal of Clinical Endocrinology and Metabolism·Bing-Kun ChenCheryl A Conover

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