Identification of intact oxidation products of glycerophospholipids in vitro and in vivo using negative ion electrospray iontrap mass spectrometry.

Journal of Mass Spectrometry : JMS
Huiyong YinGinger L Milne

Abstract

Free radical-induced oxidation products of polyunsaturated fatty acids esterified to phospholipids have been implicated in a number of human diseases including atherosclerosis and neurodegenerative diseases. Some of these phospholipid oxidation products have potent biological activities and likely contribute to human pathophysiological conditions. Oxidation products have also been used as markers of oxidative stress in vivo. Identification and quantification of phospholipid oxidation products are often performed by analyzing the oxidized free fatty acid moieties after hydrolysis from the phospholipids head groups by gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-mass spectrometry (LC-MS). We now describe the definitive identification of intact oxidized products of glycerophospholipids including glycerophosphatidylcholine (GPC), glycerophosphatidylethanolamine (GPE), and glycerophosphatidylserine (GPS) in vitro and in vivo using iontrap MS. For these analyses, the negative ions of the oxidation products of phospholipids are fragmented to MS(n) and unequivocal structural characterization is obtained based on collision-induced dissociation (CID) of the sn-2 carboxylate ion. This technique overcomes the need ...Continue Reading

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Citations

Aug 25, 2011·Chemical Reviews·Huiyong YinNed A Porter
Aug 19, 2011·Chemical Reviews·Ginger L MilneL Jackson Roberts
Nov 9, 2011·Translational Research : the Journal of Laboratory and Clinical Medicine·Pilar Codoñer-FranchEulalia Alonso-Iglesias
Aug 13, 2011·Biochimica Et Biophysica Acta·Valerie B O'Donnell
Nov 22, 2013·Journal of Mass Spectrometry : JMS·A ReisM R M Domingues
Feb 20, 2015·Antioxidants & Redox Signaling·Corinne M Spickett, Andrew R Pitt
Feb 22, 2012·Biochimica Et Biophysica Acta·Ana Reis, Corinne M Spickett
Sep 15, 2010·Free Radical Research·Corinne M SpickettNeven Zarkovic
May 5, 2016·The Journal of Clinical Endocrinology and Metabolism·Cyndya A ShibaoNada A Abumrad
Nov 5, 2013·Analytical Chemistry·Whitney L StuttsRichard A Yost
Aug 28, 2021·Diagnostics·Lyudmila V Bel'skayaVictor K Kosenok

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