PMID: 6411853Sep 1, 1983Paper

Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity

The Journal of Experimental Medicine
C F NathanB Y Rubin

Abstract

Human blood mononuclear leukocytes stimulated with toxoplasma antigen, concanavalin A, mezerein plus lentil lectin, or staphylococcal enterotoxin A secreted a factor (macrophage-activating factor, or MAF) that enhanced the capacity of human macrophages to release H2O2 and to kill toxoplasmas. The same lymphoid supernatants contained IFN gamma but not IFN alpha or IFN beta. The MAF activity of six of seven unfractionated supernatants was completely eliminated by a monoclonal antibody that neutralizes IFN gamma, and MAF in the remaining supernatant was almost completely neutralized. Native IFN gamma partially purified by two independent protocols to specific activities of 1 X 10(6) and 10(7) U/mg protein was enriched in MAF activity at least as much as in antiviral activity. The capacity of macrophages to secrete H2O2 after incubation in partially purified native IFN gamma (mean peak stimulation, 8.8-fold) was greater than with unpurified lymphokines (3.8-fold) and sometimes equaled or exceeded the capacity of freshly harvested monocytes. The MAF activity of the partially purified native IFN gamma preparations was abolished by monoclonal anti-IFN gamma. Finally, IFN gamma of greater than 99% estimated purity was isolated (at Gene...Continue Reading

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Citations

May 1, 1993·Journal of Bioluminescence and Chemiluminescence·R S TenglerF E Maly
Jan 1, 1987·Cancer Metastasis Reviews·M Shearer, J Taylor-Papadimitriou
Feb 1, 1991·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C S Subauste, J S Remington
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Sep 8, 2012·Seminars in Immunopathology·Christopher D DupontChristopher A Hunter
Nov 20, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W WujcickaD Nowakowska
Oct 30, 2008·Cell Stress & Chaperones·Brian Henderson, Samantha Henderson
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Sep 3, 1985·Journal of Immunological Methods·R Peck

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