Identification of interleukin-13 receptor alpha2 chain overexpression in situ in high-grade diffusely infiltrative pediatric brainstem glioma.

Neuro-oncology
Bharat H JoshiUS Pediatric Brain Tumor Consortium

Abstract

Human malignant glioma cell lines and adult brain tumors overexpress high levels of interleukin-13 receptor alpha2 chain (IL-13Ralpha2). Because the IL-13Ralpha2 chain is an important target for cancer therapy and prognosis for patients with brainstem glioma (BSG) remains dismal, we investigated the expression of this receptor in specimens of diffusely infiltrative pediatric BSG relative to normal brain tissue. Twenty-eight BSG specimens and 15 normal brain specimens were investigated for IL-13Ralpha2 protein expression by immunohistochemical analysis (IHC) using two different antibodies in two different laboratories. Highly sensitive Q-dot-based IHC and in situ hybridization (ISH) assays were also developed to identify IL-13Ralpha2 protein and RNA in these specimens. The results were evaluated independently in two laboratories in a blinded fashion. By Q-dot IHC or a standard IHC assay, 17 of 28 (61%) tumor specimens showed modest to strong staining for IL-13Ralpha2, while 15 normal brain tissue samples showed weak expression for IL-13Ralpha2 protein. Significant interrater agreement between the two laboratories was seen in the assessment of IL-13Ralpha2 intensity. High-level IL-13Ralpha2 RNA expression was detected in tumor sa...Continue Reading

References

Jan 1, 1996·Pediatric Neurosurgery·A M KaplanR J Packer
Aug 8, 1997·The Journal of Biological Chemistry·N I ObiriR K Puri
Sep 23, 1997·Biochemical and Biophysical Research Communications·T MurataR K Puri
Sep 10, 1998·Journal of the National Cancer Institute·M A SmithR Simon
Dec 16, 1998·International Journal of Molecular Medicine·T MurataR K Puri
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·S R HusainR K Puri
Jun 12, 2002·Current Treatment Options in Oncology·K J CohenJ Glod
Aug 15, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Mark T JenningsViolet Shen
Dec 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Koji KawakamiRaj K Puri
Mar 19, 2003·The Journal of Experimental Medicine·Nancy WoodMichael J Grusby
Jul 25, 2003·Drug News & Perspectives·B H JoshiR K Puri
Dec 3, 2003·Journal of Neuro-oncology·Syed R Husain, Raj K Puri
Apr 5, 2005·Cancer Research·Shaik Ohidar RahamanS Jaharul Haque
May 17, 2006·Technology in Cancer Research & Treatment·Mitomu KioiRaj K Puri
May 19, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Gregory J A MuradRussell R Lonser
Oct 10, 2006·Vitamins and Hormones·Bharat H JoshiRaj K Puri
Mar 1, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Sandeep KunwarUNKNOWN Cintredekin Besudotox Intraparenchymal Study Group

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Citations

Jan 13, 2012·Current Oncology Reports·Amulya A Nageswara Rao, Roger J Packer
Mar 13, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christine E BrownMichael C Jensen
Nov 19, 2008·British Journal of Neurosurgery·P A LeachI D Kamaly-Asl
Oct 31, 2015·Molecular Therapy : the Journal of the American Society of Gene Therapy·Giedre KrenciuteStephen Gottschalk
Aug 17, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·J Russell GeyerJames M Boyett
Jul 28, 2004·The Annals of Thoracic Surgery·Bryce D BesethRaj K Puri
May 18, 2010·International Journal of Cancer. Journal International Du Cancer·Toshio FujisawaRaj K Puri
May 6, 2014·Journal of Neuro-oncology·Nathan J Robison, Mark W Kieran
Nov 23, 2017·Cellular and Molecular Life Sciences : CMLS·Michaël H MeelEsther Hulleman
Jan 1, 2012·American Society of Clinical Oncology Educational Book·Nathan J Robison, Mark W Kieran
Apr 13, 2017·Frontiers in Oncology·Danielle H LapinDavid S Ziegler
Jun 11, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christine E BrownMichael C Jensen

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