Identification of intracellular target proteins of the calcium-signaling protein S100A12

European Journal of Biochemistry
Takashi HatakeyamaRyoji Kobayashi

Abstract

In this report, we have focused our attention on identifying intracellular mammalian proteins that bind S100A12 in a Ca2+-dependent manner. Using S100A12 affinity chromatography, we have identified cytosolic NADP+-dependent isocitrate dehydrogenase (IDH), fructose-1,6-bisphosphate aldolase A (aldolase), glyceraldehyde-3-phosphate dehydrogenese (GAPDH), annexin V, S100A9, and S100A12 itself as S100A12-binding proteins. Immunoprecipitation experiments indicated the formation of stable complexes between S100A12 and IDH, aldolase, GAPDH, annexin V and S100A9 in vivo. Surface plasmon resonance analysis showed that the binding to S100A12, of S100A12, S100A9 and annexin V, was strictly Ca2+-dependent, whereas that of GAPDH and IDH was only weakly Ca2+-dependent. To localize the site of S100A12 interaction, we examined the binding of a series of C-terminal truncation mutants to the S100A12-immobilized sensor chip. The results indicated that the S100A12-binding site on S100A12 itself is located at the C-terminus (residues 87-92). However, cross-linking experiments with the truncation mutants indicated that residues 87-92 were not essential for S100A12 dimerization. Thus, the interaction between S100A12 and S100A9 or immobilized S100A12 ...Continue Reading

References

Nov 1, 1995·The International Journal of Biochemistry & Cell Biology·A FilipekW Leśniak
Nov 15, 1993·Biochemical and Biophysical Research Communications·M WatanabeH Hidaka
Apr 1, 1993·Cell Calcium·D B Zimmer, J G Dubuisson
Jan 12, 1996·The Journal of Biological Chemistry·W S MailliardD D Schlaepfer
Apr 18, 1996·Nature·J HeierhorstB E Kemp
Feb 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M GarbugliaR Donato
Aug 5, 1996·Biochemical and Biophysical Research Communications·E C IlgC W Heizmann
Nov 12, 1996·Biochemical and Biophysical Research Communications·T Y ChristovaJ Ovádi
Oct 17, 1998·The EMBO Journal·T A MillwardB A Hemmings
Jan 27, 1999·Biochimica Et Biophysica Acta·G SorciR Donato
May 25, 1999·Protein Expression and Purification·K YamashitaR Kobayashi
Jul 27, 2000·The Journal of Biological Chemistry·J C DeloulmeJ Baudier
Dec 8, 2000·Biochimica Et Biophysica Acta·M GarbugliaR Donato
Jan 3, 2001·Acta Crystallographica. Section D, Biological Crystallography·O V MorozI B Bronstein
Feb 24, 2001·Protein Science : a Publication of the Protein Society·T D KimJ Kim
Jul 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·G S JacksonJ Collinge
Feb 22, 2002·Acta Crystallographica. Section D, Biological Crystallography·O V MorozI B Bronstein
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
May 7, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Claus W HeizmannBeat W Schäfer
Nov 26, 2003·The Journal of Biological Chemistry·Miki OkadaRyoji Kobayashi

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Citations

Apr 30, 2008·Amino Acids·Jens Pietzsch, Susan Hoppmann
Jan 6, 2009·The Journal of Biological Chemistry·Chantrakorn ChampaiboonMark C Herzberg
Jul 26, 2006·Mediators of Inflammation·Kazuya KishimotoKazuhide Hasegawa
Apr 24, 2009·BMC Biochemistry·Olga V MorozIgor B Bronstein
Jul 28, 2012·Current Molecular Medicine·R DonatoC L Geczy
Jan 5, 2010·Oxidative Medicine and Cellular Longevity·Rebecca V VinceLeigh A Madden
Aug 12, 2014·International Journal of Molecular Sciences·Xinyun LiShuhong Zhao
Aug 24, 2010·Biochimie·Romy M HeilmannJörg M Steiner
Mar 19, 2008·Biophysical Chemistry·A F GarciaA P U Araújo
Sep 18, 2008·The FEBS Journal·Naofumi MiwaSatoru Kawamura
Sep 18, 2008·The FEBS Journal·Anne C Rintala-DempseyGary S Shaw
Jul 14, 2010·Biophysical Chemistry·Werner W StreicherGeorge I Makhatadze
Jan 6, 2009·Biochimica Et Biophysica Acta·Estelle LeclercClaus W Heizmann
Oct 28, 2005·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Feb 6, 2019·The FEBS Journal·Donald E SprattGary S Shaw
Feb 7, 2020·Biological Reviews of the Cambridge Philosophical Society·Jacques BaudierGary S Shaw
Nov 10, 2005·The Journal of Biological Chemistry·Carlos A BuscagliaVictor Nussenzweig
Mar 19, 2020·Gastroenterology Research and Practice·Alexandre CarvalhoJennifer A Gaddy
May 11, 2006·The Biochemical Journal·Liliana Santamaria-KisielGary S Shaw
Feb 17, 2009·Biochemistry·Nicole M MarlattGary S Shaw
Nov 3, 2010·Colloids and Surfaces. B, Biointerfaces·Maria D L OliveiraCesar A S Andrade

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