Identification of iron-loaded ferritin as an essential mitogen for cell proliferation and postembryonic development in Drosophila

Cell Research
Sheng Li

Abstract

Animal cells require extrinsic cues for growth, proliferation and survival. The propagation of Drosophila imaginal disc cells in vitro, for example, requires the supplementation of fly extract, the composition of which remains largely undefined. Here I report the biochemical purification of iron-loaded ferritin as an active ingredient of fly extract that is required for promoting the growth of clone 8 imaginal disc cells. Consistent with an essential role for iron-loaded ferritin in cultured cells, overexpression of ferritin or addition of iron in a nutrient-poor diet increases animal viability and body weight, promotes cell proliferation, and shortens the duration of postembryonic development. Conversely, overexpression of dominant-negative ferritin or addition of iron chelator causes the opposite effects. Ferritin mutant flies arrest development at the first-instar larval stage with a severe starvation phenotype reminiscent of that seen in starved larvae. I conclude that iron-loaded ferritin acts as an essential mitogen for cell proliferation and postembryonic development in Drosophila by maintaining iron homeostasis and antagonizing starvation response.

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Citations

May 21, 2013·Nutrients·Konstantinos MandilarasFanis Missirlis
Feb 11, 2016·International Journal of Molecular Sciences·Abraham Rosas-ArellanoFanis Missirlis
Dec 10, 2015·Journal of Proteomics·Manisha MishraPradhyumna Kumar Singh
Oct 1, 2013·IUBMB Life·Xiaona Tang, Bing Zhou
Apr 5, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Teodoro Coba de la PeñaFederico M Winkler
Jan 26, 2018·The Journal of Experimental Biology·Carlos Tejeda-GuzmánFanis Missirlis
Feb 2, 2018·Frontiers in Physiology·Shavonn R WhitenZach N Adelman
Dec 19, 2018·Wiley Interdisciplinary Reviews. Developmental Biology·Arthur LuhurAndrew C Zelhof
Oct 16, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiaona Tang, Bing Zhou
Apr 25, 2020·Microorganisms·Ana C MoreiraMaria Salomé Gomes
Jun 19, 2013·Metallomics : Integrated Biometal Science·Lucía GutiérrezFanis Missirlis
Oct 14, 2019·Journal of Insect Science·Dawn L GeiserJoy J Winzerling
Feb 21, 2021·The Journal of Biological Chemistry·Xiaojuan WangWei Liu
Apr 24, 2018·Biochimica Et Biophysica Acta. General Subjects·Xudong WangBing Zhou

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