PMID: 9173895Apr 1, 1997Paper

Identification of isoforms of the exocytosis-sensitive phosphoprotein PP63/parafusin in Paramecium tetraurelia and demonstration of phosphoglucomutase activity

The Biochemical Journal
Karin HauserHelmut Plattner

Abstract

PP63 (parafusin) is a 63 kDa phosphoprotein which is very rapidly (within 80 ms) dephosphorylated (to P63) during triggered trichocyst exocytosis; this occurs selectively in exocytosis-competent Paramecium tetraurelia strains. In the present work, two cDNAs coding for PP63/parafusin have been isolated, one of which is a new isoform. These isoforms are 99.6% identical and are derived from two different genes. Similarity searches revealed 43-51% identity of the deduced amino acid sequences with known phosphoglucomutases from yeast and mammals. The sequences of two proteolytic peptides obtained from PP63/parafusin isolated from Paramecium are identical to parts of the amino acid sequence deduced from the major cDNA. The major cDNA was mutated from the macronuclear ciliate genetic code into the universal genetic code and expressed in Escherichia coli. The recombinant protein shows the same biochemical and immunological characteristics as the (P)P63/parafusin originally isolated from Paramecium. It has the same specific phosphoglucomutase activity as phosphoglucomutase from chicken muscle. We also show that recombinant P63-1 parafusin 1 is a substrate of an endogenous casein kinase from Paramecium, as is the originally isolated P63/...Continue Reading

Citations

Sep 5, 2013·The Journal of Eukaryotic Microbiology·Helmut Plattner
Jan 7, 1998·The Journal of Cell Biology·N D Chilcoat, A P Turkewitz
Jan 29, 2000·The Journal of Eukaryotic Microbiology·C KungR Kissmehl
Oct 22, 2015·Biological Reviews of the Cambridge Philosophical Society·Helmut Plattner
Sep 24, 2002·Journal of Cell Science·Roland KissmehlJean Cohen
Jun 24, 2000·Biochimie·L VayssiéJ Cohen
Nov 4, 2004·Biochemical and Biophysical Research Communications·Koichi TakahashiTatsuya Ingi
Feb 8, 2002·European Journal of Cell Biology·S H MatthiesenB H Satir
Aug 23, 2011·European Journal of Cell Biology·Li LiuBirgit H Satir

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