Abstract
The aim of the present study was to investigate the key gene network in fracture healing. The dataset GSE45156 was downloaded from the Gene Expression Omnibus. Differentially expressed genes (DEGs) were identified using the linear models for microarray data package of Bioconductor. Subsequently, Gene Ontology (GO) functional and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were conducted for DEGs in day 2 and 6 fractured samples via the Database for Annotation, Visualization and Integrated Discovery. Furthermore, protein‑protein interactions (PPIs) of DEGs were analyzed and a PPI network was constructed. A total of 774 and 1,172 DEGs were identified in day 2 and 6 fractured samples, respectively, compared with unfractured controls. Of the DEGs in day 2 and 6 fractured samples, various upregulated DEGs, including protein kinase C α (Prkca) and B‑cell lymphoma antagonist/killer 1 were significantly enriched in GO terms associated with cell death, and certain downregulated DEGs, including fms‑related tyrosine kinase 1 (Flt1), nitric oxide synthase 3 (Nos3), bone morphogenetic protein 4 (Bmp4) and Notch1 were enriched in GO terms associated with angiogenesis. Furthermore, a series of downregulated DEGs were e...Continue Reading
References
Aug 3, 1992·FEBS Letters·P A MarsdenT Michel
Aug 15, 1997·The Journal of Biological Chemistry·L HaatajaN Heisterkamp
Sep 23, 1997·Biochemical and Biophysical Research Communications·A SawanoM Shibuya
Aug 14, 1998·Calcified Tissue International·E M ShoreF S Kaplan
Jan 26, 1999·Clinical Orthopaedics and Related Research·A H Reddi
Feb 13, 2001·Endocrinology·K E ArmourS H Ralston
May 15, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Gang LiDavid Marsh
Mar 5, 2003·Journal of Cellular Biochemistry·Louis C GerstenfeldThomas A Einhorn
Jan 15, 2005·Lymphatic Research and Biology·Grzegorz SzczesnyMarzanna Zaleska
Feb 8, 2005·The Journal of Biological Chemistry·Masuhiro NobtaAkira Yamaguchi
Sep 9, 2006·Journal of Cellular Biochemistry·Xinmin LiJames T Ryaby
Nov 23, 2006·Acta Pharmacologica Sinica·Lin LinChun-yan Zhou
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Juwen ShenHualiang Jiang
Sep 6, 2007·Genome Biology·Da Wei HuangRichard A Lempicki
Oct 19, 2007·Biochemical and Biophysical Research Communications·Xinmin LiJames T Ryaby
May 30, 2008·Chinese Journal of Traumatology = Zhonghua Chuang Shang Za Zhi·Tong-wei ChuLiu Da-wei Liu
Jun 24, 2008·Bone·Zana KalajzicIvo Kalajzic
Jun 6, 2009·Journal of Cell Science·Amra Hajdo-MilasinovicJohn G Collard
Apr 16, 2010·Annals of the New York Academy of Sciences·Chao WanThomas L Clemens
Aug 5, 2010·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·J K R S MagalhaesM Glogauer
Nov 11, 2010·Methods in Molecular Biology·Michael KohlBettina Warscheid
Apr 15, 2011·Injury·Richard Marsell, Thomas A Einhorn
Nov 16, 2011·Stem Cells·Danka GrcevicIvo Kalajzic
Jun 23, 2012·International Journal of Oral Science·Yan-Qi YangA Bakr M Rabie
Jun 6, 2013·Journal of Dental Research·H RoguljicI Kalajzic
Jul 12, 2013·PloS One·Michael I DishowitzKurt D Hankenson
Nov 6, 2013·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Brya G MatthewsIvo Kalajzic
Mar 29, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Maisa O Al-SebaeiLouis C Gerstenfeld
Oct 30, 2014·Nucleic Acids Research·Damian SzklarczykChristian von Mering
Dec 21, 2014·Bone·Cuicui WangMatthew J Hilton
Feb 1, 2015·Neuroscience·J ZhuX Liu