PMID: 11920592Mar 29, 2002Paper

Identification of known and novel immunogenic T-cell epitopes from tumor antigens recognized by peripheral blood T cells from patients responding to IL-2-based treatment

International Journal of Cancer. Journal International Du Cancer
Carmen ScheibenbogenDirk Schadendorf

Abstract

In previous studies CD8+ T cells specific for melanocyte antigens have been frequently found in melanoma patients responding to interleukin-2 (IL-2)-based therapies. In our study we analyzed the suitability of using circulating T cells from melanoma patients with clinical response after IL-2-based therapy to identify novel T-cell epitopes from defined tumor antigens. Using unstimulated peripheral blood mononuclear cells and the interferon-gamma (IFN-gamma) ELISPOT assay, we studied CD8(+) T-cell responses against 5 peptides from the tumor antigen tyrosinase (Tyr) selected by epitope prediction using an HLA-A1-binding computer algorithm. T cells specifically secreting IFN-gamma in response to 3 of these 5 peptides, namely, Tyr (454-463), Tyr (146-156) and Tyr (243-251), could be detected in 4 of 4 HLA-A1-positive patients with clinical response. In contrast, no T-cell responses against these peptides were seen in 6 HLA-A1-positive melanoma patients with progressive disease and in 8 healthy subjects. We could generate specific cytotoxic T lymphocytes (CTL) against Tyr (454-463) using peptide-pulsed autologous dendritic cells as antigen-presenting cells. The induced CTLs efficiently killed melanoma cells that express HLA-A1 and ty...Continue Reading

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Citations

Apr 17, 2008·Cancer Immunology, Immunotherapy : CII·Lynn WenandyMads Hald Andersen
Apr 2, 2003·Journal of Immunological Methods·Rika DraenertBruce D Walker
Jul 6, 2007·Leukemia·J H Kessler, C J M Melief
Sep 5, 2006·Journal of Translational Medicine·Mads Hald AndersenPer thor Straten
Jan 20, 2004·Journal of Clinical Laboratory Analysis·Nayara Delgado AndreMaria Angelica Ehara Watanabe
Feb 13, 2009·The Journal of Investigative Dermatology·Rikke Baek SørensenMads Hald Andersen
Mar 11, 2004·The Journal of Investigative Dermatology·Mads Hald AndersenPer thor Straten
Sep 12, 2006·Trends in Molecular Medicine·Maurizio ProvenzanoFrancesco M Marincola
Dec 18, 2012·Oncoimmunology·Shamaila MunirMads Hald Andersen
Sep 16, 2004·Blood·Mads Hald AndersenPer Thor Straten
Apr 4, 2009·Journal of Immunotherapy·Anne Marie AsemissenCarmen Scheibenbogen
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mads Hald AndersenPer thor Straten
Jan 1, 2014·Oncoimmunology·Stine Kiaer LarsenMads Hald Andersen
Dec 26, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anne Marie AsemissenCarmen Scheibenbogen
Aug 4, 2004·Cancer Research·Mads Hald AndersenJürgen C Becker

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