Identification of lamina V and VII interneurons presynaptic to adrenal sympathetic preganglionic neurons in rats using a recombinant herpes simplex virus type 1

Neuroscience
H A ClarkeL C Weaver

Abstract

Although indirect evidence suggests that the control of sympathetic preganglionic neurons is mediated to a great extent through interneurons, little is known about the location, morphology or neurotransmitter phenotype of such interneurons. This limitation seriously impedes our understanding of spinal synaptic circuits crucial to control of arterial pressure and other visceral functions. We used a highly neurotropic, minimally cytopathic recombinant herpes simplex virus type-1 to study spinal "sympathetic" interneurons labelled by trans-synaptic transport of the virus from the adrenal gland in rats. Approximately 120-320 infected neurons/rat were identified by immunocytochemical detection of the viral antigen. We distinguished between virus-infected preganglionic neurons and infected interneurons by (i) their location within the spinal laminae, (ii) their size and shape and (iii) the presence or absence of immunoreactivity for the acetylcholine-synthesizing enzyme, choline acetyltransferase, a marker of sympathetic preganglionic neurons. Virus-labelled sympathetic preganglionic neurons were found within the known spinal preganglionic nuclei. Non-cholinergic, virus-labelled neurons were located throughout lamina VII and in the v...Continue Reading

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Citations

Jul 16, 2003·Journal of Pediatric Surgery·A J A HollandC M Mellis
Dec 12, 2001·Neuroscience·J E JacobA Brown
Mar 1, 2008·Clinical and Experimental Pharmacology & Physiology·Ann K GoodchildPaul M Pilowsky
Jun 16, 2009·Journal of Chemical Neuroanatomy·Ida J Llewellyn-Smith
Jun 14, 2005·The Journal of Comparative Neurology·Ida J Llewellyn-SmithJanet R Keast
Jun 16, 2007·The Journal of Comparative Neurology·Ida J Llewellyn-SmithAnn M Schreihofer
Jul 15, 2015·Neurorehabilitation and Neural Repair·Christopher R WestAndrei V Krassioukov
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·D R MarshL C Weaver

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