Identification of LETM1 as a marker of cancer stem-like cells and predictor of poor prognosis in esophageal squamous cell carcinoma

Human Pathology
Zhao-Ting YangYan-Hua Xuan

Abstract

Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) is closely related to the occurrence and development of malignant tumors. This study discusses the expression of LETM1 in esophageal squamous cell carcinoma (ESCC) and its association with cancer stem-like cells (CSC). We used immunohistochemistry in 166 ESCC tissue samples, as well as Western blot and immunofluorescent methods in ESCC cell lines, to study the role of LETM1 and its association with CSC in ESCC. The expression of LETM1 was significantly higher in ESCC, and it was closely related to the primary tumor stage and clinical stage. LETM1 expression was significantly associated with lower overall survival and disease-free survival. In addition, the protein expression of LETM1 and CSC markers was higher in TE11 and ECG10 than in other ESCC cell lines. Moreover, the expression of LETM1 positively correlated with LSD1, CD44, and OCT4. Immunofluorescence revealed that LETM1 costained with CD44 and OCT4 in ECG10. The expression of LETM1 was associated with not only HIF-1α but also higher microvessel density and tumor-associated macrophage infiltration. Furthermore, LETM1 significantly correlated with cyclinD1 and pAkt. High expression of LETM1 indicates poor p...Continue Reading

Citations

Oct 16, 2019·Oncology Letters·Qian WuZhifeng Ning
Jan 16, 2019·International Journal of Molecular Sciences·Qi-Tong Lin, Peter B Stathopulos
May 28, 2020·International Journal of Molecular Sciences·Megan NoblePeter B Stathopulos
Feb 24, 2020·Experimental and Molecular Pathology·Haoyue LiYanhua Xuan
Apr 4, 2021·Cancers·Leila JahangiriSuzanne D Turner
Apr 6, 2021·Frontiers in Physiology·Gayathri K NatarajanWai-Meng Kwok
Oct 27, 2021·Physiological Reviews·Joanne F Garbincius, John W Elrod

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.