PMID: 7021482Jan 1, 1981Paper

Identification of living spermatogenic cells of the mouse by transillumination-phase contrast microscopic technique for 'in situ' analyses of DNA polymerase activities

Histochemistry
Martti Parvinen, N B Hecht

Abstract

The stages of spermatogenesis can be identified in freshly isolated, unstained adult mouse seminiferous tubules using a transillumination method. Late acrosome- and maturation phase spermatids, arranged in bundles at stages XII-VI give rise to a spotty transillumination pattern. Before spermiation, these cells form a continuous layer on the top of the seminiferous epithelium, recognized by a strong homogeneous central light absorption in the freshly isolated seminiferous tubules at stages VII and VIII. Other stages have a pale light absorption pattern. The accurate determination of the developmental stages of the germ cells was based on the morphology of the developing acrosomic system and of the nuclei of the spermatids, as revealed by phase contrast microscopy. Using this procedure, the activity levels of DNA polymerases alpha and beta have been studied by autoradiography of squash preparations. Using endogenous templates, assay conditions that differentiate between the solubilized DNA polymerases alpha and beta in vitro, were used to distinguish between these activities in situ in different stages of mouse spermatogenesis. Except in very late spermatids shortly before spermiation, DNA polymerases alpha and beta were detectab...Continue Reading

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Citations

Dec 1, 1995·Microscopy Research and Technique·L Johnson
Aug 1, 1990·Journal of Reproductive Immunology·S MaddocksP Pöllänen
May 27, 2006·Theriogenology·L JohnsonW L Scrutchfield
Nov 14, 2003·Biology of Reproduction·Marjut OtalaLeo Dunkel
Feb 3, 2005·Asian Journal of Andrology·Eduardo Bustos-ObregonGustavo F Gonzales
Jun 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Bruce T LahnDavid C Page
Feb 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Igor MartianovIrwin Davidson
Feb 15, 2002·Human Fertility : Journal of the British Fertility Society·Martti Parvinen, Sami Ventelä
Jul 1, 1983·Journal of Steroid Biochemistry·J P MatherC W Bardin
Sep 30, 2005·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Ling-Hong LiMari S Golub
Apr 15, 2004·Genesis : the Journal of Genetics and Development·Ming ZhaoMarvin L Meistrich
May 21, 2003·International Journal of Andrology·Tarja-Leena PenttiläJorma Paranko
Sep 1, 1988·Cytometry·J ToppariG S diZerega
Oct 8, 2005·Fertility and Sterility·Ekaterina V ZubkovaBernard Robaire
Jan 12, 2002·The American Journal of Pathology·Virve PentikäinenLeo Dunkel
Apr 25, 2000·Biology of Reproduction·P M Navolanic, A O Sperry
Mar 1, 2002·Biology of Reproduction·Carlos Egydio de CarvalhoYoshitake Nishimune
Aug 10, 2004·Molecular Human Reproduction·Laura SuomalainenVirve Pentikäinen
Sep 11, 2001·The Journal of Clinical Endocrinology and Metabolism·V PentikäinenL Dunkel
Jun 8, 2000·The Journal of Clinical Endocrinology and Metabolism·V PentikäinenL Dunkel
Feb 10, 1999·The American Journal of Physiology·V PentikäinenL Dunkel

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