Identification of lncRNAs involved in biological regulation in early age-related macular degeneration

International Journal of Nanomedicine
Wei ZhuYan Wu

Abstract

Age-related macular degeneration (AMD) is one of the most common causes of adult blindness in developed countries. However, the role of long noncoding RNAs (lncRNAs) in the development and progression of early AMD is unclear. We established the lncRNA profile of early AMD by reannotation of microarrays from the gene expression omnibus database. Quantitative real-time polymerase chain reaction was used to determine the expression of selected lncRNAs. The expression profiles of 9 cases of AMD and 7 controls were studied. A total of 266 differentially expressed genes (DEGs) were detected (94 upregulated and 172 downregulated). Among all the DEGs, 64 were lncRNAs. Advanced bioinformatics analyses demonstrated that differentially expressed lncRNAs could play significant roles in visual perception, sensory perception of light stimulus, and cognition. The pathway analyses showed that the two most significantly influenced Kyoto Encyclopedia of Genes and Genomes pathways were those of phototransduction and purine metabolism. By the analyses of the key lncRNAs, it was found that RP11-234O6.2 was downregulated in the aging retinal pigment epithelium (RPE) cellular model. Exogenous RP11-234O6.2 treatment led to increased cell viability and...Continue Reading

Citations

May 28, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Qi ZhuBo Yang
Jun 12, 2021·Journal of Inflammation Research·Wencui WanYu Zhu
Jul 29, 2021·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Heba AleneziNicole Carnt

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Datasets Mentioned

BETA
GSE50195
AF131216.6
AC000124.1

Methods Mentioned

BETA
PCRs
transfection
PCR
flow cytometry
RNA-Seq

Software Mentioned

R
Pathview
GraphPad
Bioconductor
Prism GraphPad
clusterProfiler R package
DAVID
LIMMA
Cytoscape
RIsearch

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