PMID: 2110829Mar 6, 1990Paper

Identification of localized redox states in plant-type two-iron ferredoxins using the nuclear Overhauser effect

Biochemistry
L B DugadI Bertini

Abstract

The homonuclear Overhauser effect (NOE), in conjunction with nonselective spin-lattice relaxation measurements, has been employed to assign the contact-shifted resonances for the reduced form of two typical plant-type two-iron ferredoxins from the algae Spirulina platensis and Porphyra umbilicalis. These results demonstrate that the NOE should have broad general applicability for the assignments and electronic structural elucidation of diverse subclasses of paramagnetic iron-sulfur cluster proteins. NOE connectivities were detected only among sets of resonance exhibiting characteristically different deviations from Curie behavior, providing strong support for the applicability of the spin Hamiltonian formulation for the NMR properties of the antiferromagnetically coupled iron clusters [Dunham, W. R., Palmer, G., Sands, R. H., & Bearden, A. J. (1971) Biochim. Biophys. Acta 253, 373-384; Banci, L., Bertini, I., & Luchinat, C. (1989) Struct. Bonding (in press)]. The geminal beta-methylene protons for the two cysteines bound to the iron(II) center were clearly identified, as well as the C alpha H and one C beta H for each of the cysteines bound to the iron(III). The identification of the iron bound to cysteines 41 and 46 as the iro...Continue Reading

References

Oct 1, 1976·European Journal of Biochemistry·P W AndrewB G Haslett
Dec 11, 1975·Biochimica Et Biophysica Acta·R E AndersonH L Crespi
Jan 1, 1985·Comparative Biochemistry and Physiology. A, Comparative Physiology·A B Thomson
Jan 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·M PoeA S Pietro
Dec 7, 1971·Biochimica Et Biophysica Acta·W R DunhamA J Bearden
Jun 1, 1972·Archives of Biochemistry and Biophysics·I Salmeen, G Palmer
Jan 22, 1971·Biochemical and Biophysical Research Communications·J D GlicksonM Poe

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Citations

Jul 19, 2000·Proteins·A V GrinbergR Bernhardt
Apr 1, 1996·Protein Science : a Publication of the Protein Society·T A LyonsT C Pochapsky
Aug 1, 1993·Journal of Bioenergetics and Biomembranes·V D SledT Ohnishi
Feb 1, 1994·Journal of Bioenergetics and Biomembranes·H M HoldenJ L Markley
Jan 1, 1996·Biochimie·T C PochapskyG Ratnaswamy
Jul 17, 1998·Current Opinion in Chemical Biology·M K Johnson
Dec 23, 2008·The Journal of Physical Chemistry. a·Derrick R J KollingSergei A Dikanov
Jan 15, 1993·European Journal of Biochemistry·I BertiniM Vasak
Aug 31, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lucia BanciMaria-Eirini Pandelia
Dec 23, 1998·FEBS Letters·C RomeroA Donaire
Aug 9, 2005·Biochemical and Biophysical Research Communications·Swati PrasadSamaresh Mitra
May 27, 2015·Chembiochem : a European Journal of Chemical Biology·Sigrun RumpelWolfgang Lubitz
Feb 28, 2007·Biophysical Journal·Tiffany E WeksbergB Montgomery Pettitt
Feb 4, 2005·Biopolymers·Saurabh Kumar ShakyaVolkhard Helms
Mar 26, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Ekaterina S RyabovaEbbe Nordlander
Feb 18, 2005·The Journal of Biological Chemistry·Vadim Yu KuznetsovIrina F Sevrioukova
Mar 24, 2018·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Lucia BanciMario Piccioli
Sep 9, 2018·The Journal of Chemical Physics·Brennan J WalderPhilip J Grandinetti
Feb 24, 2001·Inorganic Chemistry·V TangoulisD P Kessissoglou
Mar 28, 2002·Journal of the American Chemical Society·Timothy E MachonkinJohn L Markley
Jan 6, 2018·Biochemistry·Ziliang MaoDelmar S Larsen
Nov 21, 2002·Journal of the American Chemical Society·Alexander DikiyStefano Ciurli

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