Identification of low molecular weight allergens of American cockroach and production of monoclonal antibodies

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
C H WuS F Luo

Abstract

Previous studies have identified two allergic fractions (Cr-PI and Cr-PII) from crude American cockroach extract, from which the 72- and 78-kilodalton (kD) components of Cr-PI have been identified as major allergens. The objective of this study was to identify important allergens in Cr-PII and produce monoclonal antibodies. Sera of cockroach-sensitive atopic patients were examined for IgE binding to Cr-PII proteins and monoclonal antibodies were generated by fusion between spleen cells of BALB/c mice immunized with Cr-PII and FO cells. Immunoblotting revealed that eight allergenic compounds in Cr-PII are able to bind specific IgE. Allergens of 18, 28, 32 and 45 kD bound 16.7% (2/12), 100% (12/12), 83.3% (11/12), and 100% (12/12) of the atopic sera tested, respectively. Cell fusion resulted in three stable subclones secreting monoclonal antibodies, and none of the monoclonal antibodies recognized any epitopes of German cockroach and Cr-PI of American cockroach. One monoclonal antibody reacted strongly with the 28- and 32-kD allergens of Cr-PII, and two monoclonal antibodies were able to bind the 18- and 12-kD components of Cr-PII with similar epitope specificities. All the epitopes defined by monoclonal antibodies are recognized...Continue Reading

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Citations

May 10, 2003·Journal of Immunological Methods·Wei-Jen TsaiChiou-Ying Yang
Nov 5, 2002·Molecular Immunology·Chii-Huei WuChing-Yuan Tseng
Jun 9, 2006·Environmental Health Perspectives·Nitat SookrungPramuan Tapchaisri
Feb 25, 2014·Current Allergy and Asthma Reports·L Karla ArrudaAnna Pomés
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Oct 4, 2003·Immunology and Allergy Clinics of North America·Rohit K Katial
May 8, 2018·Molecular Immunology·Steffie de Gier, Kitty Verhoeckx

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