PMID: 9161881Mar 1, 1997Paper

Identification of lymphocyte 5-HT3 receptor subtype and its implication in fish T-cell proliferation

Immunology Letters
J P MeynielP Deschaux

Abstract

In the present study, we identified the serotonergic receptor of type 3 (5-HT3) on the lymphocytes of a teleost fish, Oncorhynchus mykiss. In the pharmacological studies on the binding of [3H]serotonin to membrane receptor sites, 2-methyl-5-HT, an agonist of 5-HT3 receptors, displaced the binding of [3H]serotonin to fish lymphocytes, indicating the presence of 5-HT3 receptors on these cells. The known antagonists of the mammalian 5-HT3 receptor, ICS-205-930 and metoclopramide, failed to displace [3H]serotonin binding to lymphocytes during the period of association equilibrium (8 min); however, these antagonists progressively displaced [3H]serotonin binding from 10 to 40 min of incubation. These results suggest that fish 5-HT3 lymphocyte receptors may differ pharmacologically from mammalian receptors. As mammalian 5-HT3 receptors are coupled with Na+ inward movements, we undertook a study on Na+ influx by using SBFI/AM, a fluorescent probe. In SBFI/AM loaded fish lymphocytes, 2-methyl-5-HT leaked Na+ inward movements. Prior incubation of lymphocytes for 30 min in the presence of 5-HT3 antagonists, ICS-205-930, metoclopramide and MDL-72222, curtailed significantly the Na+ influx evoked by 2-methyl-5-HT, demonstrating that Na+ is ...Continue Reading

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Citations

May 28, 2013·Developmental and Comparative Immunology·Giuseppe Scapigliati
Sep 25, 1999·Journal of Neuroimmunology·N A Khan, J P Poisson
Sep 26, 2007·Brain, Behavior, and Immunity·J E Duffy-Whritenour, J T Zelikoff
Mar 19, 1999·Brain, Behavior, and Immunity·R Mössner, K P Lesch
Mar 8, 2008·Critical Reviews in Toxicology·N Kreke, D R Dietrich
Sep 1, 2004·European Journal of Pharmacology·András PalotásJános Kálmán

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