Identification of lysine decarboxylase as a mammalian cell growth inhibitor in Eikenella corrodens: possible role in periodontal disease

Microbial Pathogenesis
M LevineH Q Pan

Abstract

The pathogenesis of inflammatory periodontal disease was studied by examining the mechanism of HeLa and HL60 cell growth inhibition by cell-free saline-soluble extracts of Eikenella corrodens and bacterial plaque. Previous studies identified a protein (p80) as causing growth inhibition by E. corrodens extracts. After purification by two-dimensional SDS-PAGE, p80 was digested with protease lysC. Amino acid sequences were obtained and backtranslated for use as PCR primers. A 5840 nucleotide sequence containing a lysine decarboxylase gene was obtained from a Sau3 A1 genomic library of E. corrodens DNA. Lysine decarboxylase activity was present at physiologic pH in the E. corrodens extracts containing p80, and also in bacterial plaque. Both extracts caused growth inhibition by depleting lysine from cell culture media through conversion to cadaverine. Adding lysine, or immune goat IgG to a peptide derived from the active site sequence of E. corrodens lysine decarboxylase, retarded lysine depletion and growth inhibition. epsilon-Amino caproic acid specifically enhanced lysine decarboxylase activity at the low lysine concentration in HL60 cell culture media, and also increased the growth inhibition. Thus, lysine decarboxylases such as...Continue Reading

References

Sep 1, 1989·Oral Microbiology and Immunology·A Progulske-FoxS C Holt
Nov 1, 1987·Journal of Periodontal Research·J D Walters
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J P Tam
Oct 1, 1986·Tandlaegebladet·R Attström
May 1, 1986·Journal of Clinical Periodontology·M A Listgarten
Oct 1, 1985·Journal of Dental Research·M BickelP Giovannini
Jan 1, 1973·Journal of Periodontal Research·M LevineG C Cowley
Dec 1, 1974·Archives of Oral Biology·M LevineD K Mason
Feb 1, 1982·Analytical Biochemistry·A P PhanH M Lenhoff
Jan 1, 1994·Journal of Periodontal Research·J I Salonen
Feb 1, 1997·Archives of Oral Biology·G S GriffithsM A Curtis
Apr 1, 1997·Journal of Periodontal Research·M T PöllänenJ I Salonen
Jul 1, 1997·Journal of Bacteriology·Y KikuchiY Kamio
Jan 1, 1997·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·R NiedermanS Kashket
Mar 12, 1998·Journal of Clinical Periodontology·S S SocranskyR L Kent
Jun 1, 1987·Oral Microbiology and Immunology·M LevineD L Schwartzott

❮ Previous
Next ❯

Citations

Jul 15, 2011·Omics : a Journal of Integrative Biology·Mahmoud A GhannoumPatrick M Gillevet
Dec 23, 2011·International Journal of Dentistry·Martin Levine
Nov 26, 2002·Journal of Clinical Periodontology·M LevineS S Socransky
Aug 23, 2006·Oral Microbiology and Immunology·A D HaffajeeS S Socransky
Aug 26, 2006·Periodontology 2000·Ricardo P TelesSigmund S Socransky
Jun 25, 2010·International Journal of Cosmetic Science·N Lourith, M Kanlayavattanakul
Jun 26, 2015·Journal of Periodontology·Zsolt LohinaiMartin Levine
Sep 14, 2016·Journal of Periodontology·Martin LevineRicardo P Teles
Nov 27, 2007·Journal of Dental Research·G M ZakharyM Levine
Nov 26, 2008·Journal of Chemotherapy·F MeriggiA Zaniboni

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.