Identification of M1 and M2 muscarinic acetylcholine receptors in the cat carotid body chemosensory system

Neuroscience
Machiko ShirahataR S Fitzgerald

Abstract

The carotid body is a major arterial chemoreceptor that senses low O2 tension, high CO2 tension and low pH in the arterial blood. It is generally believed that neurotransmitters, including acetylcholine (ACh), participate in the genesis of afferent neural output from the carotid body and modulate the function of chemoreceptor cells (glomus cells). Previous pharmacological studies suggest that M1 and M2 muscarinic ACh receptors (mAChRs) are involved in these processes. This study was designed to demonstrate the presence and localization of M1 and M2 mAChRs in the carotid body and in the petrosal ganglion of the cat. Since DNA sequences of the cat M1 and M2 mAChRs were not known, we first determined partial DNA sequences. These sequences and deduced amino acid sequences highly resembled those of human and the rat. Subsequent reverse transcription-polymerase chain reaction (RT-PCR)analysis has demonstrated that mRNAs for M1 and M2 mAChRs are present in the carotid body and the petrosal ganglion of the cat. Immunohistochemistry has indicated that the localization of these receptors appears different. Immunoreactivity for M1 mAChR was strong in nerves in the carotid body. Nerve endings positively stained for M1 mAChR appear to inner...Continue Reading

References

Oct 25, 1991·Brain Research·B G DingerS J Fidone
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·A I Levey
Mar 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiC R Taylor
Nov 5, 1999·Brain Research·R S FitzgeraldH Y Wang
Jul 18, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S R ShiC R Taylor
Feb 1, 2002·Brain Research·Hay-Yan Jack Wang, Robert S Fitzgerald
Oct 3, 2002·Annual Review of Genetics·Stephen J O'BrienNaoya Yuhki
Sep 10, 1952·Acta Physiologica Scandinavica·S LANDGRENY ZOTTERMAN

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Citations

May 28, 2005·Respiratory Physiology & Neurobiology·Aida Bairam, John L Carroll
May 11, 2010·Respiratory Physiology & Neurobiology·Carolina R SotoJulio Alcayaga
Feb 8, 2007·Respiratory Physiology & Neurobiology·Machiko ShirahataRobert S Fitzgerald
Nov 13, 2014·Frontiers in Physiology·Ana R NunesEmília C Monteiro
Jun 6, 2019·Physiology·Tymoteusz ZeraJulian F R Paton
Jun 23, 2018·Frontiers in Physiology·Andrea PorzionatoRaffaele De Caro
Aug 23, 2020·International Journal of Molecular Sciences·Hayyaf S AldossaryAndrew P Holmes
Feb 12, 2021·Physiological Reviews·Rodrigo IturriagaVirend K Somers

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