PMID: 701374Oct 1, 1978Paper

Identification of macrophage external membrane proteins and their possible role in cell adhesion

The Journal of Cell Biology
E PearlsteinV Defendi

Abstract

Starch-activated mouse peritoneal macrophages (STpMAC) plated on plastic demonstrate the adhesive properties typical for activated pMAC: attaching as round cells and, within 15 min, spreading out with marginal membrane ruffles. These attached STpMAC were labeled by lactoperoxidase-catalysed 125I surface iodination, sodium dodecyl-sulfate-lysed, and the lysates electrophoresed on polyacrylamide gels which were examined by autoradiography. The STpMAC morphological phenotype correlates with the labeling of a particular protein (195,000, estimated mol wt). Normal pMAC (NpMAC), from unstimulated mice, do not spread and do not display the 195,000 band. Both pMAC band patterns, including the 195,000 band, are relatively resistant to trypsin digestion, as is pMAC adhesion itself trypsin-resistant. Neither class of pMAC exhibits fibronectin (Cell Adhesion Factor, LETS protein) which is a component in the adhesive matrix of cells forming trypsin-sensitive monolayers. When pMAC are tested against antifibronectin antibody, these cells do not give immunofluorescent staining. In summary, two functions in pMAC adhesion, enzyme resistance and the ability to spread, appear related to molecular properties distinctive for pMAC surface protein.

References

Aug 1, 1975·The Journal of Experimental Medicine·Z Werb, S Gordon
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Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·L M WahlG R Martin

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Citations

Jan 1, 1981·Archives of Biochemistry and Biophysics·W A Davies, T P Stossel
Mar 1, 1980·The Journal of Experimental Medicine·K AlitaloA Vaheri
Dec 1, 1980·The Journal of Experimental Medicine·E Remold-O'Donnell
Apr 1, 1983·The Journal of Experimental Medicine·C Darte, H Beaufay
Jan 1, 1986·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·B GayS Sanchez-Teff
Sep 1, 1990·Scandinavian Journal of Immunology·T A Springer
Apr 1, 1984·The Journal of Pathology·A J D'Ardenne, J O McGee
Jun 1, 1982·European Journal of Clinical Investigation·C H DuboisP R Mahieu
Jan 12, 1988·Biochemistry·E Remold-O'Donnell, B Savage
Aug 1, 1989·Scandinavian Journal of Immunology·M Patarroyo, M W Makgoba

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