Identification of macrophage migration inhibitory factor (MIF) in human skin and its immmunohistochemical localization

FEBS Letters
Tadamichi ShimizuW Sakamoto

Abstract

The presence and tissue localization of macrophage migration inhibitory factor (MIF) in human skin were examined. Reverse transcription-polymerase chain reaction analysis revealed that MIF mRNA was expressed in both surgically obtained normal human epidermis and primary cultured human keratinocytes. The expression of MIF was further confirmed by Western blot analysis, which demonstrated a single band at about 12.5 kDa using a polyclonal antibody against human recombinant MIF. Immunohistochemical studies showed that MIF existed in human epidermis, especially in the basal layer. The pathophysiological role of MIF in human skin remains undefined; however, the present results indicate that MIF may play an important role in immunity, inflammation and cellular differentiation of epidermal cells.

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Citations

May 29, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S KobayashiS Todo
Nov 12, 2005·Histochemistry and Cell Biology·Tatsuya OhkawaraJun Nishihira
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Jan 22, 2005·Journal of Dermatological Science·Tadamichi Shimizu
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