Identification of major histocompatibility complex class I C molecule as an attachment factor that facilitates coronavirus HKU1 spike-mediated infection

Journal of Virology
Che-Man ChanKwok-Yung Yuen

Abstract

Human coronavirus HKU1 (HCoV-HKU1) is a recently discovered human coronavirus associated with respiratory tract infections worldwide. In this study, we have identified the major histocompatibility complex class I C molecule (HLA-C) as an attachment factor in facilitating HCoV-HKU1 spike (S)-mediated infection. HCoV-HKU1 S pseudotyped virus was assembled using a human immunodeficiency virus type 1-derived reporter virus harboring the human codon-optimized spike of HCoV-HKU1. We identified human alveolar epithelial A549 cells as the most susceptible cell line among those tested to infection by HCoV-HKU1 S pseudotypes. A549 cells were shown to bind purified soluble HCoV-HKU1 S(1-600) glycopeptide. To search for the functional receptor for HCoV-HKU1, an A549 cDNA expression library was constructed and transduced into the nonpermissive, baby hamster kidney cells line BHK-21. Transduced cells that bind soluble HCoV-HKU1 S(1-600) glycoprotein with C-terminal FLAG were sorted. Sequencing of two independent clones revealed cDNA inserts encoding HLA-C. Inhibition of HLA-C expression or function by RNAi silencing and anti-HLA-C antibody decreased HCoV-HKU1 S pseudotyped virus infection of A549 cells by 62 to 65%, whereas pretreatment of c...Continue Reading

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Citations

Jun 1, 2009·Viruses·Patrick C Y WooKwok-Yung Yuen
Jul 6, 2020·International Journal of Immunogenetics·Kay PoultonBrendan Clark
Jul 13, 2020·International Journal of Immunogenetics·Brendan Clark, Kay Poulton
Nov 11, 2020·SN Comprehensive Clinical Medicine·Charu SonkarHem Chandra Jha
Apr 2, 2021·Frontiers in Microbiology·Roger FrutosChristian A Devaux
Mar 17, 2020·International Journal of Antimicrobial Agents·Christian A DevauxDidier Raoult
Jun 1, 2021·The Journal of Biological Chemistry·John P Evans, Shan-Lu Liu
Jun 15, 2021·Frontiers in Pharmacology·Cheorl-Ho Kim
Jul 3, 2021·International Journal of Molecular Sciences·Valeria De PasqualeLuigi Michele Pavone
Jul 24, 2021·Human Immunology·Samaneh EbrahimiGhasem Solgi

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