Identification of major histocompatibility complex class I C molecule as an attachment factor that facilitates coronavirus HKU1 spike-mediated infection

Journal of Virology
Che-Man ChanKwok-Yung Yuen


Human coronavirus HKU1 (HCoV-HKU1) is a recently discovered human coronavirus associated with respiratory tract infections worldwide. In this study, we have identified the major histocompatibility complex class I C molecule (HLA-C) as an attachment factor in facilitating HCoV-HKU1 spike (S)-mediated infection. HCoV-HKU1 S pseudotyped virus was assembled using a human immunodeficiency virus type 1-derived reporter virus harboring the human codon-optimized spike of HCoV-HKU1. We identified human alveolar epithelial A549 cells as the most susceptible cell line among those tested to infection by HCoV-HKU1 S pseudotypes. A549 cells were shown to bind purified soluble HCoV-HKU1 S(1-600) glycopeptide. To search for the functional receptor for HCoV-HKU1, an A549 cDNA expression library was constructed and transduced into the nonpermissive, baby hamster kidney cells line BHK-21. Transduced cells that bind soluble HCoV-HKU1 S(1-600) glycoprotein with C-terminal FLAG were sorted. Sequencing of two independent clones revealed cDNA inserts encoding HLA-C. Inhibition of HLA-C expression or function by RNAi silencing and anti-HLA-C antibody decreased HCoV-HKU1 S pseudotyped virus infection of A549 cells by 62 to 65%, whereas pretreatment of c...Continue Reading


Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R VlasakP Palese
Sep 1, 1984·Transplantation·A S DaarP J Morris
Jan 1, 1993·Advances in Experimental Medicine and Biology·B DelmasH Laude
Mar 1, 1993·Immunological Investigations·A R Collins
Oct 23, 1998·Advances in Experimental Medicine and Biology·A F KolbS G Siddell
Oct 23, 1998·Advances in Experimental Medicine and Biology·D B Tresnan, K V Holmes
Apr 28, 1999·Trends in Microbiology·S UgoliniQ J Sattentau
Jul 10, 1999·Immunological Reviews·L C Norkin
Feb 13, 2001·Virology·T M Gallagher, M J Buchmeier
Nov 21, 2001·British Medical Bulletin·P R Clapham, A McKnight
Dec 4, 2003·Biochemical and Biophysical Research Communications·Xiaodong XiaoDimiter S Dimitrov
Dec 13, 2003·The Journal of Biological Chemistry·Swee Kee WongMichael Farzan
Mar 3, 2004·Journal of Virology·Edward B Thorp, Thomas M Gallagher
Apr 2, 2004·Cellular Microbiology·Patricia G Spear
Oct 22, 2004·Proceedings of the National Academy of Sciences of the United States of America·Scott A JeffersKathryn V Holmes
Oct 29, 2005·Journal of Virology·Cornelis A M de HaanPeter J M Rottier
Nov 4, 2005·The Journal of Infectious Diseases·Patrick C Y WooKwok-Yung Yuen
Feb 1, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Astrid VabretFrançois Freymuth
Apr 1, 2006·Glycoconjugate Journal·Christel Schwegmann-Wessels, Georg Herrler
May 18, 2006·Emerging Infectious Diseases·Frank EsperJeffrey S Kahn
Jun 8, 2006·Journal of Clinical Microbiology·Susanna K P LauKwok-yung Yuen
Oct 14, 2006·Advances in Experimental Medicine and Biology·Stefan PöhlmannHeike Hofmann
Feb 28, 2007·Virology Journal·Victor C ChuGary R Whittaker
Oct 14, 2008·Experimental Biology and Medicine·Che-Man ChanKwok-Yung Yuen

❮ Previous
Next ❯


Jun 1, 2009·Viruses·Patrick C Y WooKwok-Yung Yuen
Jul 6, 2020·International Journal of Immunogenetics·Kay PoultonBrendan Clark
Jul 13, 2020·International Journal of Immunogenetics·Brendan Clark, Kay Poulton
Nov 11, 2020·SN Comprehensive Clinical Medicine·Charu SonkarHem Chandra Jha
Apr 2, 2021·Frontiers in Microbiology·Roger FrutosChristian A Devaux
Mar 17, 2020·International Journal of Antimicrobial Agents·Christian A DevauxDidier Raoult
Jun 1, 2021·The Journal of Biological Chemistry·John P Evans, Shan-Lu Liu
Jun 15, 2021·Frontiers in Pharmacology·Cheorl-Ho Kim
Jul 3, 2021·International Journal of Molecular Sciences·Valeria De PasqualeLuigi Michele Pavone
Jul 24, 2021·Human Immunology·Samaneh EbrahimiGhasem Solgi

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.