Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes

Journal of Chromatography. a
James G CliftonDjuro Josic

Abstract

For proteomic analysis, plasma membranes of rat hepatocellular carcinoma Morris hepatoma 7777 were selectively solubilized according to the previously developed method [D. Josic, K. Zeilinger, Methods Enzymol. 271 (1996) 113-134]. If the Triton X100 insoluble pellet is subsequently extracted, several proteins can be solubilized. These proteins can be classified in two groups according to their molecular size. The proteins with apparent molecular weights in SDS-PAGE between 70 and 75 kDa belong to the first group. Smaller proteins, with apparent molecular weights between 30 and 45 kDa, are members of the second group. The main protein of higher molecular weight was also found in the Triton X100 insoluble extract from normal rat liver plasma membranes. This protein was identified as Annexin A6. The proteins from the second group are practically absent in the Triton X100 insoluble extract from rat liver. These proteins are present in relatively high concentrations in plasma membranes of Morris hepatoma 7777. Both groups of detergent-insoluble proteins from Morris hepatoma 7777 were further analyzed with SELDI-TOF and LC electrospray ionization mass spectrometry. From the first group, Annexin A6, together with two other integral pl...Continue Reading

References

Feb 1, 1978·European Journal of Biochemistry·R Tauber, W Reutter
Nov 6, 1992·Science·C E Creutz
Jun 22, 1994·Biochimica Et Biophysica Acta·C E TagoeJ H Walker
Jul 1, 1994·Trends in Genetics : TIG·P D Smith, S E Moss
Jan 1, 1996·Methods in Enzymology·D Josic, K Zeilinger
Jun 27, 1997·Biochimica Et Biophysica Acta·V Gerke, S E Moss
Apr 18, 1998·The Biochemical Journal·C Schmitz-PeifferT J Biden
Mar 2, 1999·Biochemical and Biophysical Research Communications·A MessinaV De Pinto
Sep 7, 2000·The Journal of Cell Biology·E B Babiychuk, A Draeger
Dec 8, 2000·Biochimica Et Biophysica Acta·J König, V Gerke
Feb 16, 2002·European Journal of Biochemistry·Laura D Zajchowski, Stephen M Robbins
Mar 28, 2002·Physiological Reviews·Volker Gerke, Stephen E Moss
Aug 2, 2002·Biochemical and Biophysical Research Communications·Shin-ichi MiyakeMasaya Tohyama
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Leonard J FosterMatthias Mann
Sep 10, 2003·Molecular Biology of the Cell·Marci E SchanerBranimir I Sikic

❮ Previous
Next ❯

Citations

Jun 16, 2010·Molecular & Cellular Proteomics : MCP·Noelle M Griffin, Jan E Schnitzer
Oct 26, 2013·Future Oncology·Chunmei GuoMing-Zhong Sun
Oct 17, 2009·Proteomics·Stuart J Cordwell, Tine E Thingholm
May 4, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Chunmei GuoMing-Zhong Sun
Feb 19, 2014·Molecular and Cellular Neurosciences·Jens WeingartenWalter Volknandt
Jun 28, 2011·Electrophoresis·Marijana RucevicDjuro Josic
Apr 21, 2007·Analytical and Bioanalytical Chemistry·Ming-Hui YangMing-Chen Wang
Jul 27, 2007·Proteomics·Djuro Josic, James G Clifton
Jun 8, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Huimin HeYingchun Hou
Apr 24, 2014·Proteomes·Melanie LaßekWalter Volknandt
Sep 22, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·James G CliftonDjuro Josic

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.