Identification of metabolic engineering targets for improving glycerol assimilation ability of Saccharomyces cerevisiae based on adaptive laboratory evolution and transcriptome analysis

Journal of Bioscience and Bioengineering
Kazuki KawaiTakashi Hirasawa

Abstract

Glycerol, a by-product of biodiesel production, has been utilized as a raw material for bioproduction. Saccharomyces cerevisiae, which has been used as a host microorganism for bioproduction, possesses the metabolic pathways for glycerol assimilation, but it cannot grow on glycerol as a carbon source. In this study, we identified metabolic engineering targets to improve the glycerol assimilation ability of S. cerevisiae based on adaptive laboratory evolution experiments using serial transfer of culture on glycerol and transcriptome analysis of the evolved cells using RNA-sequencing. The transcriptome data revealed that the upregulation of genes related to the tricarboxylic acid (TCA) cycle and oxidative phosphorylation contributed to the increased specific growth rate on glycerol during adaptive evolution. Furthermore, genes related to the pentose phosphate pathway were downregulated. Based on these observations, we identified metabolic engineering targets for improving glycerol assimilation. Overexpression of HAP4, which encodes one of the subunits of the Hap2p/3p/4p/5p transcription factor complex involved in the upregulation of the TCA cycle genes, or disruption of RIM15, which encodes a protein kinase related to the transcr...Continue Reading

Citations

Feb 9, 2020·Applied and Environmental Microbiology·Guoxing ZhuJianrong Wu
Oct 4, 2020·Trends in Biotechnology·Gregory GuirimandVincent Courdavault
May 11, 2021·The Journal of General and Applied Microbiology·Taiji YuzawaTakashi Hirasawa

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