Identification of metabolic pattern and bioactive form of resveratrol in human medulloblastoma cells

Biochemical Pharmacology
Xiao-Hong ShuJia Liu

Abstract

Cancer preventive reagent trans-resveratrol is intracellularly biotransformed to different metabolites. However, it is still unclear whether trans-resveratrol exerts its biological effects directly or through its metabolite(s). This issue was addressed here by identifying the metabolic pattern and the bioactive form of resveratrol in a resveratrol-sensitive human medulloblastoma cell line, UW228-3. The cell lysates and condition media of UW228-3 cells with or without 100 microM resveratrol treatment were analyzed by HPLC and LC/MS which revealed (1) that resveratrol was chemically unstable and the spontaneous generation of cis-resveratrol reduced resveratrol's anti-medulloblastoma efficacy and (2) that resveratrol monosulfate was the major metabolite of the cells. To identify the bioactive form of resveratrol, a mixture-containing approximately half fraction of resveratrol monosulfate was prepared by incubating trans-resveratrol with freshly prepared rat brain lysates. Medulloblastoma cells treated by 100 microM of this mixture showed attenuated cell crisis. The overall levels of the three brain-associated sulfotransferases (SULT1A1, 1C2 and 4A1) were low in medulloblastoma cells in vivo and in vitro in comparison with that in ...Continue Reading

References

Jul 19, 2001·Nature·R A Dixon
Aug 17, 2002·Thrombosis Research·Beata Olas, Barbara Wachowicz
Aug 27, 2003·Nature·Konrad T HowitzDavid A Sinclair
Nov 5, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shazib Pervaiz
Nov 19, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Nancy E LiyouMichael E McManus
Aug 31, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Thomas WalleU Kristina Walle
Jan 15, 2005·Trends in Molecular Medicine·Silvia Marino
Apr 20, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Moammir Hasan AzizNihal Ahmad
Oct 26, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Donggeng WangWenying Liu
May 30, 2006·Nature Reviews. Drug Discovery·Joseph A Baur, David A Sinclair
Jun 20, 2006·Explore : the Journal of Science and Healing·DeAnn LiskaDavid S Jones
Jun 30, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yazen Alnouti, Curtis D Klaassen
Oct 20, 2006·Biochemical Pharmacology·Kyung Jin WooTaeg Kyu Kwon
Nov 14, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David J BoocockWilliam P Steward
Feb 9, 2007·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Allan LançonNorbert Latruffe
Apr 12, 2007·PLoS Biology·Abdellah Allali-HassaniCheryl H Arrowsmith
Sep 19, 2007·The Journal of Pharmacology and Experimental Therapeutics·Brett BursteinStanley Nattel
Feb 22, 2008·Current Drug Metabolism·Julian LindsayShu-Feng Zhou
Mar 14, 2008·Journal of Separation Science·Laura MercoliniMaria Augusta Raggi
Jun 24, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Balachandar VenkatesanGoutam Ghosh Choudhury
Jan 28, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Emily D SalmanCharles N Falany
Mar 17, 2009·European Journal of Pharmacology·Yan LiUrban Lindgren
Mar 24, 2009·Free Radical Biology & Medicine·Elizabeth D Brookins DanzRebecca S Keller

❮ Previous
Next ❯

Citations

Aug 31, 2013·Cancer Chemotherapy and Pharmacology·Zheng SunJia Liu
Nov 23, 2013·Apoptosis : an International Journal on Programmed Cell Death·Shu-Er ChowJong-Shyan Wang
Jun 15, 2011·Expert Opinion on Drug Delivery·Ana Cláudia SantosAntónio J Ribeiro
Aug 24, 2016·Cell Death Discovery·L-X ZhongH Li
Nov 9, 2011·Journal of Cellular Biochemistry·Wei YuWei Wang
Nov 8, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jana RousovaAlena Kubátová
Mar 30, 2018·International Journal of Molecular Sciences·Yi-Tian LiHong Li
Nov 23, 2019·Journal of the Science of Food and Agriculture·Bingren Tian, Jiayue Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.