Identification of metallothionein isoforms with capillary zone electrophoresis using a polyacrylamide-coated tube

Journal of Chromatography. B, Biomedical Applications
T MinamiY Okazaki

Abstract

Metallothionein (MT) isoforms from various liver tissues were separated with capillary zone electrophoresis (CZE) using a polyacrylamide-coated tube at neutral pH. The electrophoresis was performed on MT-1 and MT-2 purified from mouse, rat, rabbit and human livers. The retention times of mouse and rat MT-1 coincided, while the retention times of rabbit and human MT-1 were longer. The retention times of MT-2 purified from the four sources were the same. MT-1 and MT-2 separated more definitely with N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES)-Tris buffer (25 mM, pH 7.4) than with N-tris(hydroxymethyl)methyl-3-aminopropane sulfonic acid (TAPS)-Tris buffer (25 mM, pH 7.7) or with N-(2-acetamido)iminodiacetic acid (ADA)-Tris buffer (25 mM, pH 7.4). In addition, liver MT isoforms prepared from Zn- or Cd-administered mice could be separated.

Citations

Oct 31, 2008·Talanta·R LobinskiJ Szpunar
Oct 4, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Takeshi MinamiSeiji Ichida
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Takeshi MinamiKanenobu Kubo
Sep 15, 2005·Journal of Applied Toxicology : JAT·Sylvie Bobillier-ChaumontAlain Berthelot

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