Identification of metastasis-associated proteins in a human tumor metastasis model using the mass-mapping technique

Proteomics
Paweena KreuninSteve Goodison

Abstract

For most cancer cell types, the acquisition of metastatic ability leads to clinically incurable disease. The identification of molecules whose expression is specifically correlated with the metastatic spread of cancer would facilitate the design of therapeutic interventions to inhibit this lethal process. In order to facilitate metastasis gene discovery we have previously characterized a pair of monoclonal cell lines from the human breast carcinoma cell line MDA-MB-435 that have different metastatic phenotypes in immune-compromised mice. In this study, serum-free conditioned media was collected from the cultured monoclonal cell lines and a mass mapping technique was applied in order to profile a component of each cell line proteome. We utilized chromatofocusing in the first dimension to obtain a high resolution separation based on protein pI, and nonporous silica reverse-phase high performance liquid chromatography was used for the second dimension. Selected proteins were identified on the basis of electrospray ionization time of flight mass spectrometry (ESI-TOF MS) intact protein mapping and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) peptide mass fingerprinting. Using this appr...Continue Reading

Citations

Feb 15, 2011·Inflammatory Bowel Diseases·Alexandra I Thompson, Charlie W Lees
Feb 5, 2010·Functional & Integrative Genomics·Ivana RatkajSandra Kraljevic Pavelic
Dec 3, 2009·Analytica Chimica Acta·Andrew J Percy, David C Schriemer
Jun 10, 2008·Journal of the American Society for Mass Spectrometry·Lian ShanDavid J Anderson
May 24, 2007·Journal of Proteome Research·Paweena KreuninSteve Goodison
Jun 13, 2012·Cell Research·Mario Andres BlancoYibin Kang
Jul 19, 2005·Cancer Research·Steve GoodisonVirginia Urquidi
Apr 5, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Na YangSteven Goodison
Nov 15, 2007·Cytogenetic and Genome Research·V Urquidi, S Goodison
Nov 18, 2011·Expert Review of Proteomics·Alan A DoucetteShayla Fitzsimmons
Jun 6, 2008·Expert Review of Proteomics·Steve Goodison, Virginia Urquidi
Jul 21, 2012·Journal of Proteomics·Piia-Riitta KarhemoPirjo Laakkonen
Oct 12, 2007·Experimental Dermatology·Ien ChanJohn A McGrath
Jul 1, 2007·Proteomics. Clinical Applications·Efisio PuxedduLuciano Binaglia
Jul 29, 2005·International Journal of Cancer. Journal International Du Cancer·Marta LomnytskaSerhiy Souchelnytskyi
Jan 31, 2009·Veterinary and Comparative Oncology·B Matharoo-BallR Rees
Oct 4, 2006·Critical Reviews in Oncology/hematology·Christine H ChungDavid P Carbone
Aug 8, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Yue ChenXueyuan Xiao
Apr 16, 2005·Clinical Science·Walter KolchAndrew R Pitt
Jul 4, 2008·Journal of Proteome Research·Melkamu Getie-KebtieMichail A Alterman

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.