Identification of metE as a second target of the sRNA scr5239 in Streptomyces coelicolor

PloS One
Michael-Paul VockenhuberBeatrix Suess

Abstract

While transcriptional regulation of the primary and secondary metabolism of the model organism Streptomyces coelicolor is well studied, little is still known about the role small noncoding RNAs (sRNAs) play in regulating gene expression in this organism. Here, we report the identification of a second target of the sRNA scr5239, an sRNA highly conserved in streptomycetes. The 159 nt long sRNA binds its target, the mRNA of the cobalamin independent methionine synthase metE (SCO0985), at the 5' end of its open reading frame thereby repressing translation. We show that a high methionine level induces expression of scr5239 itself. This leads, in a negative feedback loop, to the repression of methionine biosynthesis. In contrast to the first reported target of this sRNA, the agarase dagA, this interaction seems to be conserved in a wide number of streptomycetes.

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Citations

Sep 17, 2015·Environmental Microbiology·Alejandro Arce-RodríguezVíctor de Lorenzo
Dec 30, 2017·RNA·Shoji Ohuchi, Beatrix Suess
Oct 18, 2016·Molecular Microbiology·Matthias Gimpel, Sabine Brantl
Mar 30, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Meike J SaulDieter Steinhilber
Mar 3, 2020·Frontiers in Microbiology·Franziska EngelBeatrix Suess
Aug 12, 2020·Journal of Industrial Microbiology & Biotechnology·Yongjae LeeByung-Kwan Cho
Sep 8, 2018·Microbiology Spectrum·Medha RainaCarin K Vanderpool
Oct 7, 2021·The EMBO Journal·Kavyaa VenkatKai Papenfort

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Methods Mentioned

BETA
in vitro transcription
PCR
RNAseq

Software Mentioned

RNAhybrid

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