Identification of microbes from the surfaces of food-processing lines based on the flow cytometric evaluation of cellular metabolic activity combined with cell sorting

Biofouling
Wojciech JuzwaKatarzyna Czaczyk

Abstract

In this study the design of a flow cytometry-based procedure to facilitate the detection of adherent bacteria from food-processing surfaces was evaluated. The measurement of the cellular redox potential (CRP) of microbial cells was combined with cell sorting for the identification of microorganisms. The procedure enhanced live/dead cell discrimination owing to the measurement of the cell physiology. The microbial contamination of the surface of a stainless steel conveyor used to process button mushrooms was evaluated in three independent experiments. The flow cytometry procedure provided a step towards monitoring of contamination and enabled the assessment of microbial food safety hazards by the discrimination of active, mid-active and non-active bacterial sub-populations based on determination of their cellular vitality and subsequently single cell sorting to isolate microbial strains from discriminated sub-populations. There was a significant correlation (r = 0.97; p < 0.05) between the bacterial cell count estimated by the pour plate method and flow cytometry, despite there being differences in the absolute number of cells detected. The combined approach of flow cytometric CRP measurement and cell sorting allowed an in situ ...Continue Reading

References

Dec 1, 1994·Applied and Environmental Microbiology·R G McClelland, A C Pinder
Feb 1, 1993·Journal of Clinical Microbiology·C BolletP De Micco
Aug 26, 1998·Journal of Applied Microbiology·G N CaronR A Badley
Mar 4, 2000·Applied and Environmental Microbiology·T S GunasekeraD Veal
May 3, 2000·International Journal of Food Microbiology·C W Blackburn, J D McCarthy
Sep 23, 2000·Journal of Microbiological Methods·G Nebe-von-CaronR A Badley
Sep 12, 2002·International Journal of Food Microbiology·T A McMeekin, T Ross
Feb 13, 2003·Letters in Applied Microbiology·D GarcíaR Pagán
Mar 26, 2003·Journal of Microbiological Methods·J T Keer, L Birch
Jun 20, 2003·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·Hazel M Davey
Jun 23, 2005·Critical Reviews in Food Science and Nutrition·M van der SpiegelW M F Jongen
Sep 1, 2005·Critical Reviews in Food Science and Nutrition·Ioannis S Arvanitoyannis, Athina Traikou
Aug 29, 2006·International Journal of Food Microbiology·T A McMeekinMarcel H Zwietering
Apr 5, 2008·International Journal of Food Microbiology·Mieke UyttendaeleFrank Devlieghere
Nov 17, 2009·International Journal of Food Microbiology·Arie H HavelaarBenno H Ter Kuile

Methods Mentioned

BETA
flow cytometry
FACS
PCR

Related Concepts

Cell Density
Flow Cytometry
Food Processing
Stainless Steel
Streak Plate Count
Fimbrial Adhesins
Virus Viability
Bacterial Count Measurement
Cell Count
Cell Death

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