Identification of microRNA-like RNAs in a plant pathogenic fungus Sclerotinia sclerotiorum by high-throughput sequencing

Molecular Genetics and Genomics : MGG
Jiahong ZhouJiasen Cheng


MicroRNAs are small non-coding RNAs of approximately 22 nt with important regulatory roles in post-transcriptional regulation of gene expression. To date, no miRNAs have been reported in plant pathogenic fungi. Here we used Solexa sequencing to find new miRNAs in a plant pathogenic fungus Sclerotinia sclerotiorum. 13,229,401 high-quality reads were obtained, a total of 5,645,718 reads were mapped to the S. sclerotiorum genome corresponding to 348,108 unique sRNAs. Among the sRNAs, 2 microRNA-like RNAs (milRNAs) and 42 mil-RNA candidates were identified by sequence analysis. Northern blot and RT-PCR results showed that some milRNAs and candidates are differentially expressed in sclerotial development suggesting the role of these milRNAs and candidates in this biological process. Our results provide new insights into the functional roles of small RNAs and add new resources for the study of plant pathogenic fungi.


Apr 9, 2002·Genes & Development·Caterina CatalanottoCarlo Cogoni
Jul 9, 2002·Genes & Development·Brenda J ReinhartDavid P Bartel
Aug 31, 2002·Cell·Matthew W RhoadesDavid P Bartel
Feb 20, 2003·RNA·Victor AmbrosThomas Tuschl
Apr 4, 2003·Genes & Development·Lee P LimDavid P Bartel
Jul 19, 2003·Science·James C Carrington, Victor Ambros
Dec 31, 2003·Cell·Benjamin P LewisChristopher B Burge
Mar 10, 2004·BMC Evolutionary Biology·S Blair HedgesJason L Shoe
Apr 2, 2004·Nature·Sandra K Floyd, John L Bowman
Jul 1, 2004·Development·Patrick AchardNicholas P Harberd
Jul 20, 2004·The Plant Cell·Ramanjulu Sunkar, Jian-Kang Zhu
Dec 21, 2004·Nucleic Acids Research·Sam Griffiths-JonesAlex Bateman
Mar 19, 2005·The Plant Journal : for Cell and Molecular Biology·Joonki KimChung-Mo Park
Aug 4, 2005·Nucleic Acids Research·Ramanjulu SunkarJian-Kang Zhu
Sep 6, 2005·Science·Cheng LuPamela J Green
Dec 31, 2005·Nucleic Acids Research·Sam Griffiths-JonesAnton J Enright
May 4, 2006·Annual Review of Plant Biology·Matthew W Jones-RhoadesBonnie Bartel
Jun 6, 2006·Nature·Angélique GirardMichelle A Carmell
Dec 22, 2006·Genes & Development·Ramya RajagopalanDavid P Bartel
Jul 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gert C SegersDonald L Nuss
Sep 28, 2007·Fungal Genetics and Biology : FG & B·Xiaoyan GongYouliang Peng
Mar 4, 2008·BMC Plant Biology·Ramanjulu SunkarJian-Kang Zhu
Jan 13, 2009·Cell·Changchun Xiao, Klaus Rajewsky
Jan 16, 2009·Journal of Virology·Jia Yun ZhuGunter Meister
Mar 31, 2009·FEBS Letters·Tina RathjenTamas Dalmay
May 16, 2009·Genomics·Wei-Chen LinPetrus Tang
Sep 19, 2009·Nature Reviews. Genetics·Carlo M Croce
Mar 20, 2010·Methods in Molecular Biology·Stephanie D FiedlerLane K Christenson

❮ Previous
Next ❯


Aug 5, 2014·Annual Review of Phytopathology·Arne WeibergHailing Jin
Mar 30, 2016·Seminars in Cell & Developmental Biology·Ane Sesma
Nov 26, 2015·Fungal Genetics and Biology : FG & B·Santiago Torres-Martínez, Rosa M Ruiz-Vázquez
Jul 5, 2016·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Runmao LinAiping Zheng
Dec 28, 2016·International Journal of Molecular Sciences·Anna Lukasik, Piotr Zielenkiewicz
Oct 26, 2016·Scientific Reports·Yushi LuanJun Meng
Mar 5, 2016·Mycologia·José Manuel Villalobos-EscobedoNohemí Carreras-Villaseñor
Apr 5, 2018·Science China. Life Sciences·Wen ZhangZhen Zhou
Feb 13, 2016·Microbiology and Molecular Biology Reviews : MMBR·Monika SchmollAlfredo Herrera-Estrella
Jan 24, 2019·Functional & Integrative Genomics·Summi DuttaKunal Mukhopadhyay
Mar 30, 2020·The New Phytologist·Ming XuLili Huang
May 16, 2013·Molecular Microbiology·Nohemi Carreras-VillaseñorAlfredo Herrera-Estrella
Jun 29, 2017·Annual Review of Microbiology·Santiago Torres-Martínez, Rosa M Ruiz-Vázquez
Feb 19, 2019·Genetics and Molecular Biology·Juliana S de CurcioCélia Maria de Almeida Soares
Apr 11, 2019·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Yun JinHui-Shan Guo
Jul 25, 2020·Functional & Integrative Genomics·Himanshu DubeyTilak Raj Sharma
Oct 11, 2020·Trends in Plant Science·Harriet MiddletonAbdelhak El Amrani

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.