Identification of microRNAs and their target genes in Alport syndrome using deep sequencing of iPSCs samples

Journal of Zhejiang University. Science. B
Wen-biao ChenYong Dai

Abstract

MicroRNAs (miRNAs) are a class of small RNA molecules that are implicated in post-transcriptional regulation of gene expression during development. The discovery and understanding of miRNAs has revolutionized the traditional view of gene expression. Alport syndrome (AS) is an inherited disorder of type IV collagen, which most commonly leads to glomerulonephritis and kidney failure. Patients with AS inevitably reach end-stage renal disease and require renal replacement therapy, starting in young adulthood. In this study, Solexa sequencing was used to identify and quantitatively profile small RNAs from an AS family. We identified 30 known miRNAs that showed a significant change in expression between two individuals. Nineteen miRNAs were up-regulated and eleven were down-regulated. Forty-nine novel miRNAs showed significantly different levels of expression between two individuals. Gene target predictions for the miRNAs revealed that high ranking target genes were implicated in cell, cell part and cellular process categories. The purine metabolism pathway and mitogen-activated protein kinase (MAPK) signaling pathway were enriched by the largest number of target genes. These results strengthen the notion that miRNAs and their target...Continue Reading

References

Jan 1, 1993·Kidney International·K TryggvasonT B Shows
Jan 1, 1995·Retina·K M GehrsG Zilkha
Feb 11, 1998·Ophthalmic Genetics·D J Colville, J Savige
Mar 13, 2003·Kidney International·Dale R AbrahamsonPatricia L St John
Jun 2, 2007·Néphrologie & thérapeutique·Marie-Claire GublerCorinne Antignac
Jun 16, 2007·Nephron. Clinical Practice·Paul Scott Thorner
Dec 26, 2007·Methods : a Companion to Methods in Enzymology·Markus HafnerThomas Tuschl
Mar 18, 2008·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Ke Wei ZhangJudy Savige
Aug 16, 2008·Genomics·Olena Morozova, Marco A Marra
May 9, 2009·Journal of the American Society of Nephrology : JASN·Dale R AbrahamsonPatricia L St John
May 12, 2009·New Biotechnology·Wilhelm J Ansorge
Jun 6, 2009·Bioinformatics·Ruiqiang LiJun Wang
Oct 29, 2010·Genome Biology·Simon Anders, Wolfgang Huber
Feb 18, 2011·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Jeffrey H Miner
Feb 24, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·X ZhangY Lu
May 27, 2011·American Journal of Physiology. Renal Physiology·Ruma PengalWilliam E Smoyer
Jul 26, 2011·Journal of the American Society of Nephrology : JASN·Wei QinHui Y Lan
Sep 24, 2011·Journal of Pediatric Hematology/oncology·Dong Hyun JoJeong Hun Kim
Dec 8, 2011·Toxicological Sciences : an Official Journal of the Society of Toxicology·Séin O'ConnellTara McMorrow
Jan 10, 2012·Journal of the American Society of Nephrology : JASN·Sumanth PuttaRama Natarajan
Jun 12, 2012·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Nils PfaffTobias Cantz
Sep 22, 2012·Clinical Journal of the American Society of Nephrology : CJASN·Johanna TemmeOliver Gross
Oct 24, 2012·Advances in Chronic Kidney Disease·Mariana Murea
Feb 23, 2013·Clinical and Experimental Nephrology·Jianhua HeKeizo Kanasaki
Jun 25, 2013·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Michael PohlHeidrun Rhode
Oct 8, 2013·Genomics, Proteomics & Bioinformatics·Wenwen JiaJiuhong Kang
Oct 11, 2013·International Journal of Nephrology and Renovascular Disease·Arthur Ck ChungHui Y Lan
Oct 31, 2014·Annals of Clinical and Translational Neurology·Tanel PungaAnna R Punga
Nov 12, 2014·Nature Reviews. Nephrology·Piera TrionfiniGiuseppe Remuzzi

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Citations

Jan 9, 2018·Journal of Zhejiang University. Science. B·Jun MengXiao-Yu Deng
Jan 8, 2016·Biomarker Insights·Benjamin S Freedman

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